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pCis GFPuv-SS Himar1 A7

Plasmid

#84686

Purpose

Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

Depositor

Ulrike Munderloh

Article

Munderloh et al Intracellular Pathogens II: Rickettsiale

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-CohIII-CBM-HIS-LPETGG

Plasmid

#91697

Purpose

CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

CohIII-CBM-HIS-LPETGG

Use

Tags

HIS-Tag and Sortase-Tag

Expression

Bacterial

Mutation

Cysteine deletion in CBM C63S

Promoter

T7

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX

Plasmid

#111516

Purpose

Expresses E. coli ClpX with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG358

Plasmid

#115599

Purpose

surface-displayed Ag1; medium copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Use

Tags

Expression

Bacterial

Mutation

See depositor comments below

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pM2s2AsG

Plasmid

#137924

Purpose

E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

Superfolder GFP

Use

Synthetic Biology; For use with e. coli nissle in…

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pQF

Plasmid

#48095

Purpose

Cumate-inducible expression of 3xFLAG-tagged fusion protein

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Bacterial

Mutation

Promoter

PQ5

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR2

Plasmid

#112813

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pZE27GFP5

Plasmid

#75458

Purpose

Plasmid for constitutive expression of gfp-pdt5

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

gfp-pdt#5

Use

Tags

Expression

Bacterial

Mutation

Promoter

placIq promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28GG

Plasmid

#104655

Purpose

Level 2 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pFAB808

Plasmid

#47853

Purpose

BIOFAB reporter plasmid for measuring rrnB T1 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rrnB T1 terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLTetO8

Available since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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HS_NAM-B1

Plasmid

#154064

Purpose

Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Inserts

Hygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1

Use

Cre/Lox and Synthetic Biology; Golden gate

Tags

Expression

Bacterial and Plant

Mutation

The TtNAM-B1 gene sequence was domesticated to re…

Promoter

HvHSP17, OsAct, and ZmUbi

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLz37_CB_operon_CsoS2_N1-N4

Plasmid

#140856

Purpose

Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(136)

Plasmid

#44380

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(136) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(136)

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCE38

Plasmid

#174432

Purpose

C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCE41

Plasmid

#174433

Purpose

C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMyC

Plasmid

#42192

Depositor

Annabel Parret, Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

ColE1

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HSPA9-Halo

Plasmid

#175332

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HSPA9 (HSPA9 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(120nm)-LPETGG

Plasmid

#90472

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(120nm)-LPETGG

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos9)GFP

Plasmid

#89247

Purpose

Expresses GFP with +9 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos9)-GFP

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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GST-TAX1BP1

Plasmid

#171423

Purpose

For the purifcation of TAX1BP1 protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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