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Plasmid#173080PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGAP
UseTagsmCitrineExpressionBacterialMutationmCitrine + last 10 residues of GNA1, codon optimi…PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-141
Plasmid#118557PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHCKan-INSyibp-Nef
Plasmid#134593PurposeLow Phosphate inducible NefDepositorInsertHIV Nef protein
UseTagsHis tagExpressionBacterialMutationPromoteryibDAvailable sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHEN2 F8-2
Plasmid#186033PurposeExpress VHH F8-2 with 3 c-myc tag in E. coli periplasmDepositorInsertVHH F8-2
UseTags6His and MycExpressionBacterialMutationPromoterlacAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDule-Tet2.0
Plasmid#85496PurposePlasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsertTetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
UseTagsNoneExpressionBacterialMutationY32G L65Q F108S Q109D D158S L162NPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-PO-MT-P2A
Plasmid#214899PurposeExpresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sitesDepositorInsertSPLICSFAST-PO-MT-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG323
Plasmid#115594Purposesurface-displayed NullDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAP-BAC
Plasmid#120229PurposeTAR cloning in yeast and low-copy maintenance of large constructs in E. coliDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mKOκ-EC18154
Plasmid#137077PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT100.106b
Plasmid#122601Purposeclone APOBEC1 phage by SapI Golden GateDepositorInsertAPOBEC1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneUseTagsHis6-MBP-prescissionExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-CfaC-Gyra
Plasmid#122950PurposeBacterial expression of C-inteinDepositorInsertCfaC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YBX3-Halo
Plasmid#175308PurposeProduction of HaloTag fusion proteinDepositorInsertYBX3 (YBX3 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-SUMO-MproC145A
Plasmid#172653PurposeBacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMODepositorInsertSARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis Tag and SUMOExpressionBacterialMutationCatalytic Cysteine 145 mutated to AlaninePromoterT7Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJF093
Plasmid#113323PurposeSp.pCas9-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern/pRSETB
Plasmid#51969Purposebacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imagingDepositorInsertNano-lantern
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
NW50
Plasmid#133445PurposeCovalently circularized nanodisc NW50 for studying membrane proteins and viral entry.DepositorInsertNW50
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only