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Plasmid#187382Purposechromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherryDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitC
Plasmid#122605Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p406GAL1
Plasmid#17437DepositorInsertGAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGAL1 promoter is between SacI and XbaI restrictio…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ALDH1A3
Plasmid#189750PurposeThe construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.DepositorInsertALDH1A3 (ALDH1A3 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAW014-2
Plasmid#85612PurposeMulti-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilisDepositorInsertugtP-gRNA.P395T
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT220
Plasmid#134888PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKEI-Dest
Plasmid#79784PurposeExpression plasmid - BsmBI entry sitesDepositorTypeEmpty backboneUseTags6xHisExpressionBacterialMutationPromoterpTrcAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean3
Plasmid#103973PurposePlasmid encoding mCerulean3DepositorInsertmCerulean3
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKFSS1_2
Plasmid#118227PurposeEmpty E. coli - B. burgdorferi shuttle vector; streptomycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-KSRP
Plasmid#23019DepositorInsertKSRP (KHSRP Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX NBP1 (P#415)
Plasmid#14658DepositorInsertATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H (ATP6V1H Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pU6T7
Plasmid#71462PurposeExpresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and MammalianMutationPromoterhuman U6/phage T7Available sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLS.492
Plasmid#184957PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
UseTagsExpressionBacterialMutationrpoB donor T1534C, a1/a2 length extended to 22 bpPromoterT7/lacAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme L99A WT*
Plasmid#18476DepositorInsertT4 Lysozyme (e Bacteriophage T4)
UseTagsExpressionBacterialMutationC54T C97A L99A WT*PromoterAvailable sinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp+Lux
Plasmid#26159DepositorInsertBacterial luciferase operon
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKate2
Plasmid#68441PurposeTOPO construct expressing mKate2 for generation of GMAP-compatible geneDepositorInsertmKate2
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT-MBI (JBEI-3085)
Plasmid#87950Purposethe overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).DepositorInsertMevT-MBI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only