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Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbA5a-sufCDSUB
Plasmid#195055PurposeExpresses SUF operon from B. subtilis from IPTG-inducible promoterDepositorInsertsufCDSUB
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSKBAD2
Plasmid#15335DepositorInsertDnaE C-intein
UseTagsGB1ExpressionBacterialMutationPromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
Plasmid#159548PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationG12DPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHG6
Plasmid#188254PurposeIntegrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoteract1p/actII-orf4Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
UseTagsHis tag and LAA degradation tagExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA3b61
Plasmid#58310PurposeShuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-ecfp
Plasmid#78463PurposeBroad range bacterial fluorescence labelingDepositorInserteCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-pBAD
Plasmid#54542PurposeLocalization: Protein Expression Vector , Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTags6xHis and EBFP2ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDD57
Plasmid#119882PurposeConstitutive expression of GFP+ on pAL5000/pMB1 backbone with kanamycin resistance.DepositorInsertpConstitutive_GFP+
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
TurboRFP-pBAD
Plasmid#54858PurposeLocalization: Protein Expression Vector , Excitation: 553, Emission: 574DepositorTypeEmpty backboneUseTags6xHis and TurboRFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)
Plasmid#169161PurposeTo express nsp10-nsp14 fusion protein in E. coliDepositorInsert14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSANG14-3F
Plasmid#39265DepositorTypeEmpty backboneUseTags6xHis, Alkaline phosphatase, pelB leader, and tri…ExpressionBacterialMutationPromoterT7Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pT7-7 asyn A53T
Plasmid#105727PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein A53T (SNCA Human)
UseTagsExpressionBacterialMutationA53TPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PCNA-IRES-puro2b
Plasmid#26461DepositorInsertPCNA (PCNA Human)
UseTagspEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
MYST3
Plasmid#25181PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMYST3_13 (KAT6A Human)
UseTagsHisExpressionBacterialMutationcontains catalytic region ( residues 497-780).PromoterAvailable sinceOct. 25, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18T-mini-Tn7T-Gm-Pc-sfGFP
Plasmid#199248PurposeFluorescent labelling of bacterial cells with sfGFPDepositorInsertCodon-optimized sfGFP gene
UseTagsExpressionBacterialMutationCodon optimization for XanthomonadaceaePromoterPc promoter from class III integron of Delftia a…Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB078d9
Plasmid#79207PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1+2+3); Ppro1Available sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only