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Plasmid#224370PurposeExpress EF-Tu protein for his-tagged purificationDepositorInsertEF-Tu (tufB E. coli)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-PheRSa-PheRSb
Plasmid#224364PurposeExpress PheRS protein for his-tagged purificationDepositorInsertPheRS (pheS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-AsnRS
Plasmid#224363PurposeExpress AsnRS protein for his-tagged purificationDepositorInsertAsnRS (asnS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-RRF-His6
Plasmid#224374PurposeExpress RRF protein for his-tagged purificationDepositorInsertRRF (frr E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-IF3
Plasmid#224368PurposeExpress IF3 protein for his-tagged purificationDepositorInsertIF3 (infC E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-IF1
Plasmid#224366PurposeExpress IF1 protein for his-tagged purificationDepositorInsertIF1 (infA E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-ThrRS
Plasmid#224365PurposeExpress ThrRS protein for his-tagged purificationDepositorInsertThrRS (thrS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-CKM
Plasmid#224375PurposeExpress CKM protein for his-tagged purificationDepositorInsertCKM (LOC107051134 Chicken)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-AlaRS
Plasmid#224362PurposeExpress AlaRS protein for his-tagged purificationDepositorInsertAlaRS (alaS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-NDK
Plasmid#224376PurposeExpress NDK protein for his-tagged purificationDepositorInsertNDK (ndk E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pELlR1
Plasmid#172569PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pELlR2
Plasmid#172555PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
Plasmid#101211PurposeBacterial expression plasmid for SpCas9-HF1, REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, N497A, R661A, Q695A and…PromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
Plasmid#101204PurposeBacterial expression plasmid for SpCas9∆REC3, REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368PromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mut2-RS
Plasmid#182882PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut2-RS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pediculus humanus corporis PINK1 (115 - 575)
Plasmid#110750PurposeBacterial expression of PhPINK1 (biochemistry)DepositorInsertPhum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
UseTagsN-His6-GST-3CExpressionBacterialMutationPromoterT7Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG002 (BREX type I)
Plasmid#157880PurposeExpresses the BREX type I defense systemDepositorInsertBREX type I
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
UseTagsHis-TagExpressionBacterialMutationPromoterTrcAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS mRFP
Plasmid#91765PurposeBacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)DepositorInsertmonomeric Red Fluorescent Protein1
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only