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Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
UseTags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
TriEx-4 DHX36
Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
UseTagsHistidineExpressionBacterial, Insect, and Mamm…MutationPromoterCMV ie enhancer/promoterAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-eCFP
Plasmid#78476PurposeBroad range bacterial fluorescence labeling (ECFP)DepositorInserteCFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30 PTBP1 isoform1
Plasmid#108591PurposeRecombinant His tagged protein productionDepositorInsertPTBP1 isoform 1 (PTBP1 Human)
UseTags6xHisExpressionBacterialMutationisoform 1 (isoform c)PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
TGT-His
Plasmid#138201PurposeExpresses E. Coli TGT with a C-terminal His TagDepositorInsertE Coli tRNA Guanine Transglycosylase-His
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEA183
Plasmid#187873PurposeExpresses eGFP under the virB promoter.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterAgrobacterium virB promoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-Optimized UBE1L
Plasmid#165099Purposeecoli optimized expression of GST-fusion of human Ube1LDepositorInsertUbeE1L (UBA7 Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
HSA2_His
Plasmid#202764Purposehuman serum albumin design HSA2, in pET29b with C-terminal 6His-tagDepositorInsertHSA2
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TRBP
Plasmid#50351Purposebacterial expression of TRBPDepositorInsertTRBP (TARBP2 Human)
UseTagsHis and MycExpressionBacterialMutationPromoterT7Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
UseTagsExpressionBacterialMutationPromoterGlnS (constitutive)Available sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Split 'n coexpress CUL3-RBX1
Plasmid#52293Purposebacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1DepositorUseTagsGST, IRES, and TEVExpressionBacterialMutationcontains aa 1-309, contains aa 310-768, and conta…PromoterIRES and tacAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC101-Donor
Plasmid#140630PurposeKanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.DepositorInsertLE, RE, KanR, temperature sensitive pSC101 origin of replication
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO MYC3
Plasmid#177848PurposeBacterial Expression of MYC3DepositorInsertMYC3 (MYC3 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST-KiT
Plasmid#44562Purposefor integrative inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKB233
Plasmid#170040PurposeEncodes PvirK-mNeonGreen reporterDepositorArticleInsertmNeonGreen
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvirKAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXT130_TwinStrep-SUMO-ShTnsC
Plasmid#135526PurposeProtein expression construct for ShTnsCDepositorInsertShTnsC
UseTagsTwinStrep-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_ST18
Plasmid#64844PurposeA lentiviral vector for the expression of mouse ST18 under doxycyline controlDepositorInsertST18 (St18 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS
Plasmid#52345PurposeIntegration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKateDepositorInsertmKate2
UseP1 integration vectorTagsExpressionBacterialMutationchanged V2HPromoterpGKL2 ORF10 UCSAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427T
Plasmid#81035PurposeMammalian expression of LOV I427T, a mutant with super short relaxation timeDepositorInsertLOV I427T
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427TPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only