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pOPINK-OTUD3 (OTU, aa 52-209)

Plasmid

#61412

Purpose

Expresses human OTUD3 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD3 (OTUD3 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-51 and aa 210-398.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUB1 (full-length, aa 1-271)

Plasmid

#61420

Purpose

Expresses human OTUB1 (full-length) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pGEMTEZ-Kir2.1

Plasmid

#32641

Depositor

Richard Axel, Joseph Gogos, C. Ron Yu

Article

Yu et al Neuron. 2004 May 27;42(4):553-66.

Insert

Kir2.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pSMT3-FBF2

Plasmid

#166972

Purpose

Expresses FBF-2 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575

Promoter

T7

Available since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1-HiBiT

Plasmid

#198310

Purpose

Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1-HiBiT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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AcpP pet29a C-terminal His Tag

Plasmid

#75016

Purpose

Expresses the acyl carrier protein (ACP) from the E. coli fatty acid synthase

Depositor

Michael Burkart

Article

Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

Insert

AcpP (acpP E. coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only
NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Use

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR-p300HAT

Plasmid

#157795

Purpose

Expression of sfGFP-TetR-p300HAT fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR-p300 (EP300 Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-optimizedNedd4.2d

Plasmid

#165103

Purpose

bacterial expression of a GST fusion of Human Nedd4.2d

Depositor

Arthur Haas

Article

Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

Insert

Nedd4.2d (NEDD4L Human)

Use

Tags

GST from plasmid.

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR

Plasmid

#157794

Purpose

Expression of sfGFP-TetR fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSP64T (DM#111)

Plasmid

#15030

Depositor

Douglas Melton

Article

Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

Insert

beta-globin 5' and 3' UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pHCL151

Plasmid

#134455

Purpose

TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

linker-mScarlet-I for C-terminal fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

plac

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pHCL147

Plasmid

#134454

Purpose

TetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

linker-mScarlet-I for C-terminal fusion

Use

Tags

Expression

Bacterial

Mutation

Promoter

plac

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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SNRPA_Full-length_pGEX

Plasmid

#135134

Purpose

Recombinant expression of SNRPA with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

SNRPA (SNRPA Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Feb. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGLO-GFP-3UAG

Plasmid

#82501

Purpose

Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescence Protein with 3 UAG codons

Use

Tags

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3, …

Promoter

AraBAD

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTetO-ISce1-TetO

Plasmid

#58506

Purpose

ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Tet repeats flanking ISce1 restriction site

Use

Tags

Expression

Bacterial

Mutation

Promoter

no promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only
pOPINK-OTUD1 (OTU+UIM, aa 287-481)

Plasmid

#61406

Purpose

Expresses human OTUD1 (OTU+UIM domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD1 (OTUD1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-286.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pCri_L706-antiSH2-OptoMB

Plasmid

#159935

Purpose

Opto-MB bacterial expression

Depositor

Jared Toettcher, Jose Avalos

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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