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Plasmid#35290DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterLlacO-1Available sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIT5_SL
Plasmid#173769PurposeBacterial genomic integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…TagsExpressionBacterialMutationPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL61
Plasmid#163633PurposeOptimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Gm-ecfp
Plasmid#65030PurposeGmR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET21-pelB-LaM-3
Plasmid#172769PurposeBacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFPV-mCherry-TT
Plasmid#112523PurposePrpsM driven mCherry expression with synTTDepositorInsertPrpsM mCherry TT
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-B-iRFP
Plasmid#31855Purposea phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imagingDepositorInsertiRFP
UseTagsExpressionBacterialMutationS13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…PromoteraraBADAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHN767
Plasmid#178884PurposeExpression of bacterial PNGaseDepositorInsertPeptide:N-glycosidase F
UseTagsGST, His8ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-dCas9
Plasmid#62244Purposecontrols the expression of S. pyogenes dCas9 from a Tc‐inducible PTet promoter.DepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationcatalytically inactive, D10A, H840APromoterPtetAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNCS-CyOFP
Plasmid#74278PurposeConstitutive expression of CyOFP in bacteriaDepositorInsertCyOFP
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-X(4130)B1
Plasmid#168018PurposeExpresses X(4130)B1-6xHis in bacterial cellsDepositorInsertX(4130)B1-6xHis
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAL-c5X-ELP[AV-60]
Plasmid#67012PurposeExpresses malE-ELP[AV-60] in E. coliDepositorInsertmalE-ELP[AV-60]
UseTagsHis6 and malE-Factor XaExpressionBacterialMutationPromotertacAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
LB1353 (Bcl-2 promoter, no IgH enh)
Plasmid#15393DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 with full promoter and no IgH enhancers MHS…PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
Drp1-EGFP-StrepII
Plasmid#174429PurposeExpresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteriaDepositorInsertDrp1
UseTagsEGFP-StrepIIExpressionBacterialMutationNonePromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
6MIW
Plasmid#127291PurposeBacterial expression for structure determination. May not contain entire coding region of geneDepositorInsertHUWE1 (HUWE1 Human)
UseTags6xHis-TEV (MHHHHHHSSGRENLYFQG)ExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
B-gTEMP/pRSET-b
Plasmid#188446PurposeB-gTEMP for bacterial expressionDepositorInsertB-gTEMP
UseTags6xHis-tagExpressionBacterialMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only