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Plasmid#29519DepositorUseTagsGST, His (before thrombin), and Thrombin Cut Site…ExpressionBacterialMutationPromoterAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only
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pENTR1A-SynTetOff-GFP
Plasmid#190230PurposeGateway entry vector, high-level expression of EGFP in neuronal cellsDepositorInsertEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-Tev
Plasmid#177142PurposeBacterial vector for expression of an N-terminal GST fusion of the Tev proteaseDepositorInsertTev
UseTagsGSTExpressionBacterialMutationPromoterTacAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBP-HIS2-Snf7
Plasmid#21492DepositorInsertSNF7 (SNF7 Budding Yeast)
UseTags6x His and MBPExpressionBacterialMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LolT_C41S
Plasmid#211789PurposeExpression plasmid in E. coli BL21(DE3) , which can be used for expression and purification to get protein LolT_C41SDepositorInsertlolt mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-FnCas9GFP
Plasmid#130966Purposeexpression of FnCas9-GFP in bacterial cellsDepositorInsertHA-NLS-FnCas9
UseCRISPRTags6xHis-mEGFP-TEV-HA-NLSExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM6-E12-vioABECD
Plasmid#66537PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)DepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterMutant T7 Promoter - G6 and Mutant T7 Promoter - …Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaPylRS
Plasmid#200225PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
UseTagsnoneExpressionBacterialMutationnonePromoterproK-lacO and tacIAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET.HaloTag.v7.AviTag
Plasmid#195486PurposeBacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.DepositorInsertHaloTag.v7.AviTag
UseTags6xHIS and AviTagExpressionBacterialMutationPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFNF
Plasmid#22687DepositorInsertNeo*
UseMouse TargetingTagsExpressionBacterial and MammalianMutationFRT-pPGK-pEM7-Neo*-bpA-FRTPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKI1
Plasmid#199118PurposeExpresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labellingDepositorInsertH6-SNAP-RapA
UseTags6xHis and SNAP-tagExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-WT
Plasmid#122690PurposeExpress His-tagged PKM2 (wild type) protein in bacteria.DepositorInsertPyruvate kinase M2 isoform (PKM Human)
UseTags6His-tagExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
UseTagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHJL3
Plasmid#199378PurposeEncodes dual cistronic cytosolic jGCaMP7s and mScarlet-I optimized for C. elegans expressionDepositorInsertjGCaMP7s::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
KSP_K560GFP
Plasmid#129770PurposeExpresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKSP (kif11.L Frog)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 367, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pS24·Pm-GFP
Plasmid#122594PurposeExpresses GFP under the regulatory control of Pm. Expression needs additional xyls geneDepositorInsertPm promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Synthetic_Human_H2AZ.1
Plasmid#42629DepositorInsertHuman H2AZ.1 (H2AZ1 Synthetic, Codon Optimized, Human)
UseTagsUntaggedExpressionBacterialMutationCodon OptimizedPromoterT7Available sinceMarch 21, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits