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pYFJ16-LplA(W37V) (for E. Coli expression)

Plasmid

#34838

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA W37V (lplA E. Coli)

Use

Tags

His Tag

Expression

Bacterial

Mutation

Mutated Trp37 to Val

Promoter

T5 promoter

Available since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-GDH

Plasmid

#108954

Purpose

heterologous expression of GDH of B. megaterium in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Glucose dehydrogenase IV (GDH IV)

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-SCFP3A

Plasmid

#103970

Purpose

Plasmid encoding SCFP3A

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

SCFP3A

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-His6

Plasmid

#182386

Purpose

Bacterial expression of Vesicle Nucleating peptide

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-His

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pHCL149

Plasmid

#134457

Purpose

CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

Para-popZ-rbs-H3H4-msfGFPN-CTM.

Use

Tags

Expression

Bacterial

Mutation

Promoter

Para

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre-HiBiT

Plasmid

#198318

Purpose

Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre-HiBiT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBHRSF184

Plasmid

#89482

Purpose

Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

Asparaginyl endopeptidase from Oldenlandia affinis

Use

Tags

Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINB-OTULIN (full-length, aa 1-352)

Plasmid

#61464

Purpose

Expresses human OTULIN (full-length) in E. coli.

Depositor

David Komander

Article

Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

Insert

OTULIN (OTULIN Human)

Use

Tags

His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB

Plasmid

#26107

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Type

Empty backbone

Use

Tags

His6 - Z-basic - TEV

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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APLF-c001

Plasmid

#173077

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

APLF (APLF Human)

Use

Tags

mCerulean

Expression

Bacterial

Mutation

K360-V448, codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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His-Tev-Nedd4L

Plasmid

#186806

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-Nedd4L (NEDD4L Human)

Use

Tags

His6-Tev

Expression

Bacterial

Mutation

Promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

Plasmid

#61590

Purpose

Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

Depositor

David Komander

Article

Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

Insert

TRABID (ZRANB1 Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-244 and aa 698-708.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pET26b-Granzyme A

Plasmid

#8823

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A (GZMA Human)

Use

Tags

(His)6 and Enterokinase site

Expression

Bacterial

Mutation

it mature enzyme.

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-hsFlt3L

Plasmid

#25074

Depositor

Maria Castro

Article

Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

Insert

human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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BCL2A1A

Plasmid

#42399

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

BCL2A1A (BCL2A1 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Promoter

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pOPINK-Cezanne (OTU, aa 53-446)

Plasmid

#61581

Purpose

Expresses human Cezanne (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne (OTUD7B Human)

Use

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-52 and aa 447-843.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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