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Showing: 1 - 20 of 24 results

  1. Golden GATEway Cloning Kit

    Plasmid Kit
    #1000000054
    Purpose
    Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way
    Depositor
    Joachim Wittbrodt
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. Yarrowia lipolytica Golden Gate tool kit

    Plasmid Kit
    #1000000167
    Purpose
    Yarrowia lipolytica Golden Gate tool kit for integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica.
    Depositor
    Tristan Rossignol
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  3. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid Kit
    #1000000024
    Purpose
    Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Jan Lohmann
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Takashi Yamamoto
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit
    #1000000030
    Purpose
    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.
    Depositor
    Takashi Yamamoto
    Available since
    March 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. Mobius Assembly Vector Toolkit

    Plasmid Kit
    #1000000134
    Purpose
    Mobius Assembly: Uses a two-level approach for transcriptional unit (TU) and multi-TU augmentation. Each level is comprised of four Acceptor Vectors. Compatible with Golden Gate parts
    Depositor
    Naomi Nakayama
    Available since
    March 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid Kit
    #1000000055
    Purpose
    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack
    Depositor
    Takashi Yamamoto
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. Ralstonia-GG-Kit

    Plasmid Kit
    #1000000142
    Purpose
    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques
    Depositor
    Thomas Lahaye
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  11. pDGE Dicot Genome Editing Kit

    Plasmid Kit
    #1000000084
    Purpose
    Golden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of “genome editing recipient” and “sgRNA shuttle” vectors.
    Depositor
    Johannes Stuttmann
    Available since
    Sept. 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. Komagataeibacter Tool Kit (KTK)

    Plasmid Kit
    #1000000216
    Purpose
    The Komagataeibacter Tool Kit (KTK) is a standardized modular cloning system based on Golden Gate DNA assembly.
    Depositor
    Tom Ellis
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only

  13. Yeast Secrete and Detect

    Plasmid Kit
    #1000000166
    Purpose
    Yeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.
    Depositor
    Sylvestre Marillonnet
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. MoClo Plant Parts Kit

    Plasmid Kit
    #1000000047
    Purpose
    Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation
    Depositor
    Sylvestre Marillonnet, Nicola Patron
    Available since
    April 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. T2A Split/Link TurboID Kit

    Plasmid Kit
    #1000000249
    Purpose
    The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only

  16. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design
    Depositor
    Paul Freemont
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. Fragmid

    Plasmid Kit
    #1000000237
    Purpose
    Fragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only

  18. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production
    Depositor
    Yizhi Cai
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit
    #1000000236
    Purpose
    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.
    Depositor
    Daniel Voytas
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  20. The Mammalian Toolkit

    Plasmid Kit
    #1000000180
    Purpose
    A ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.
    Depositor
    Hana El-Samad
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 24 results