158 results

1. MoClo Yeast Toolkit

   Plasmid Kit

   #1000000061

   Purpose

   96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes

   Depositor

   John Dueber

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Pro-Code Vector Kit

   Plasmid Kit

   #1000000177

   Purpose

   The Pro-Code Vector Kit is a set of 120 lentiviral vector plasmids, each with a unique protein barcode (Pro-Code) and gRNA cloning site.These plasmids can be used for CRISPR screens.

   Depositor

   Brian Brown

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

3. MoClo Tool Kit

   Plasmid Kit

   #1000000044

   Purpose

   Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators

   Depositor

   Sylvestre Marillonnet

   Available since

   April 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. CRISPaint Gene Tagging Kit

   Plasmid Kit

   #1000000086

   Purpose

   Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags

   Depositor

   Veit Hornung

   Available since

   Sept. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

5. MoClo Yeast Secretion and Display (YSD) Toolkit

   Plasmid Kit

   #1000000240

   Purpose

   The YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.

   Depositor

   Paul Young

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

6. ONE-GO biosensors

   Plasmid Kit

   #1000000224

   Purpose

   This kit consists of ten ONE vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs).

   Depositor

   Mikel Garcia-Marcos

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

7. Komagataeibacter Tool Kit (KTK)

   Plasmid Kit

   #1000000216

   Purpose

   The Komagataeibacter Tool Kit (KTK) is a standardized modular cloning system based on Golden Gate DNA assembly.

   Depositor

   Tom Ellis

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

8. C. elegans miniMos transgenesis kit

   Plasmid Kit

   #1000000031

   Purpose

   29 plasmid kit contains reagents for generating transgenic nematodes, including C. elegans and C. briggsae, using miniMos transposon. Selection with unc-119 or NeoR, PuroR.

   Depositor

   Erik Jorgensen

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

9. PRESTO-Tango Kit

   Plasmid Kit

   #1000000068

   Purpose

   Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.

   Depositor

   Bryan Roth

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

10. MoClo Plant Parts Kit

    Plasmid Kit

    #1000000047

    Purpose

    Modular Cloning (MoClo) collection contains 95 standardized parts to enable Golden Gate construction of multi-gene constructs for plant transformation

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Available since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

11. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid Kit

    #1000000236

    Purpose

    The MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.

    Depositor

    Daniel Voytas

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

12. RAS mutant clone collection

    Plasmid Kit

    #1000000089

    Purpose

    Wild-type and mutant HRAS, NRAS, KRAS4a and KRAS4b gene plasmids as Gateway entry clones

    Depositor

    Dominic Esposito

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Sybody Generation Toolbox

    Plasmid Kit

    #1000000160

    Purpose

    The Seeger Lab protocol to select synthetic nanobodies – called sybodies – against (membrane) protein targets. This collection contains all plasmids necessary for the sybody generation.

    Depositor

    Markus Seeger

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

14. Multiplex CRISPR/Cas9 Assembly System Kit

    Plasmid Kit

    #1000000055

    Purpose

    Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

    Depositor

    Takashi Yamamoto

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. TRUPATH

    Plasmid Kit

    #1000000163

    Purpose

    A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR

    Depositor

    Bryan Roth

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

16. MoClo Plant Parts II and Infrastructure Kit

    Plasmid Kit

    #1000000135

    Purpose

    Compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs, with new phytobricks and vectors to connect Modular Cloning and Gateway Cloning platforms.

    Depositor

    Johannes Stuttmann

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

17. EcoFlex MoClo Kit

    Plasmid Kit

    #1000000080

    Purpose

    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design

    Depositor

    Paul Freemont

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

18. MoClo CRISPR/Cas Toolkit for Plants

    Plasmid Kit

    #1000000159

    Purpose

    The MoClo CRISPR/Cas Toolkit for Plants includes 95 plasmids consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and dicots.

    Depositor

    Vladimir Nekrasov

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

19. EcoFlex MoClo Extension, Volume I

    Plasmid Kit

    #1000000253

    Purpose

    Use the EcoFlex extension kit (volume one) to quickly and efficiently build a wide variety of gene expression constructs using the EcoFlex-standard MoClo system.

    Depositor

    Mikhail Shapiro

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

20. GoldenBraid 2.0 Kit

    Plasmid Kit

    #1000000076

    Purpose

    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units

    Depositor

    Diego Orzaez

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only