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Showing: 1 - 20 of 149 results

  1. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit
    #1000000014
    Purpose
    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.
    Depositor
    William Hahn, David Root
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  4. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design
    Depositor
    Paul Freemont
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit
    #1000000116
    Purpose
    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
    Depositor
    Carol Gross
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  6. RAS Pathway 2.0 Clone Collection

    Plasmid Kit
    #1000000070
    Purpose
    Collection contains 180 genes in the “RAS pathway version 2.0”, a crowdsourced pathway map of the RAS signaling pathway; For use with Gateway cloning or FNLCR Combinatorial Cloning Platform
    Depositor
    Dominic Esposito
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    John Dueber
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. EasyClone 2.0 Yeast Toolkit

    Plasmid Kit
    #1000000073
    Purpose
    Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains
    Depositor
    Irina Borodina
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid Kit
    #1000000141
    Purpose
    Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9
    Depositor
    Irina Borodina
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. ONE-GO biosensors

    Plasmid Kit
    #1000000224
    Purpose
    This kit consists of ten ONE vector G protein Optical (ONE-GO) biosensor plasmids for the expression of BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs).
    Depositor
    Mikel Garcia-Marcos
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only

  11. Fragmid

    Plasmid Kit
    #1000000237
    Purpose
    Fragmid is a modular toolkit that leverages Golden Gate cloning to enable the rapid creation of CRISPR vectors compatible with various delivery approaches, enzymes, and mechanisms.
    Depositor
    John Doench
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only

  12. Minimal Antibiotic Resistance Platform (ARP)

    Plasmid Kit
    #1000000143
    Purpose
    Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.
    Depositor
    Gerard Wright
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. CIDAR MoClo Parts Kit

    Plasmid Kit
    #1000000059
    Purpose
    CIDAR Modular Cloning (MoClo) kit uses modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in E. coli
    Depositor
    Douglas Densmore
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Jan Lohmann
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. Platinum Gate TALEN Kit

    Plasmid Kit
    #1000000043
    Purpose
    Enables construction of highly-active Platinum TALENs using two-step Golden Gate cloning method. Platinum TALENs have variable TALE repeats with either +136/+63 or +153/+47 TALE scaffolds.
    Depositor
    Takashi Yamamoto
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. AID* Kit

    Plasmid Kit
    #1000000120
    Purpose
    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.
    Depositor
    Helle Ulrich
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. S. cerevisiae Advanced Gateway Destination Vectors

    Plasmid Kit
    #1000000011
    Purpose
    Yeast (S. cerevisiae) Gateway Destination Vectors for tagging and gene expression. Has variety of promoters, origin of replications, auxotrophic markers, and N- or C-terminal fusion proteins
    Depositor
    Susan Lindquist
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  18. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.
    Depositor
    Huimin Zhao
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.
    Depositor
    Markus Ralser
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. Chromoprotein kit 2.0

    Plasmid Kit
    #1000000198
    Purpose
    Chromoprotein plasmid kit that contain two new chromoprotein genes that are less toxic to E. coli so that the plasmids are more stable during growth.
    Depositor
    Anthony Forster
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 149 results