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E. coli K-12 MG1655_fLP2(yieN-trkD)

Bacterial Strain

#110242

Purpose

E. coli K-12 MG1655 with Landing Pad engineered into the yieN-trkD intergenic region. The Landing Pad consists of floxed mCherry,Chlor that are cotranscribed.

Depositor

Sriram Kosuri

Article

Urtecho et al Biochemistry. 2018 Feb 1. doi: 10.1021/a

Bacterial Resistance

Chloramphenicol

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
RF21

Bacterial Strain

#102803

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
SQ171/pCSacB/ptRNA100

Bacterial Strain

#155206

Purpose

SQ171 strain supported by pCSacB and ptRNA100 plasmids

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Bacterial Resistance

Kanamycin

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only
E. coli BL21 (DE3) ΔEntD

Bacterial Strain

#192874

Purpose

Deletion of native E. coli EntD, a 4'-PPTase required for native enterobactin synthesis. Allows expression of NRPS carrier proteins that do not harbor a phosphopantetheinyl group.

Depositor

Christian Chalut, Christophe Guilhot

Article

Chalut et al Proc Natl Acad Sci U S A. 2006 May 30;10

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only
S4246

Bacterial Strain

#156372

Purpose

Erythromycin-resistant S2060 derivative

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Bacterial Resistance

Streptomycin, tetracycline, erythromycin

Available since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only
bSLS.114

Bacterial Strain

#191530

Purpose

Contains a knockout gene in the E. coli strain BL21-AI

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Species

Escherichia coli

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only
RF10

Bacterial Strain

#62076

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
YM138

Bacterial Strain

#61953

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF1

Bacterial Strain

#61962

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF6

Bacterial Strain

#62074

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF2

Bacterial Strain

#62070

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
EcAR7

Bacterial Strain

#52055

Purpose

Strain for use in Rinehart Lab Phosphoprotein synthesis kit

Depositor

Jesse Rinehart

Article

Heinemann et al FEBS Lett. 2012 Oct 19;586(20):3716-22.

Bacterial Resistance

None

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
DH10beta F' DOT sbcC (donor strain)

Bacterial Strain

#12082

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Bacterial Resistance

Tetracycline

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
RF8

Bacterial Strain

#62075

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. asnA asnB genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
MS1

Bacterial Strain

#61960

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE hisG genes deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
BUN21/pML300 (recipient strain)

Bacterial Strain

#11675

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Bacterial Resistance

Spectinomycin

Species

Bacteriophage lambda

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
BL21(DE3) ΔserC

Bacterial Strain

#197656

Purpose

Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

Depositor

Ryan Mehl, Richard Cooley

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Bacterial Resistance

None

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only
Jun lab tunable CRISPRi strain

Bacterial Strain

#86400

Purpose

Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

Depositor

Suckjoon Jun

Article

Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

Bacterial Resistance

Spectinomycin and hygromycin

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
E. coli ER1821ΔlacI

Bacterial Strain

#141407

Purpose

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Depositor

Antal Kiss

Article

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Bacterial Resistance

None

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Showing: 81 - 100 of 114 results

Results per page:

Sort results by:

E. coli K-12 MG1655_fLP2(yieN-trkD)

RF21

SQ171/pCSacB/ptRNA100

E. coli BL21 (DE3) ΔEntD

S4246

bSLS.114

RF10

YM138

RF11

RF1

RF6

RF2

EcAR7

DH10beta F' DOT sbcC (donor strain)

RF8

MS1

BUN21/pML300 (recipient strain)

BL21(DE3) ΔserC

Jun lab tunable CRISPRi strain

E. coli ER1821ΔlacI

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