Addgene

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Strain SIJ_488

Bacterial Strain

#68246

Purpose

E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Bacterial Resistance

None

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
T-B18

Bacterial Strain

#159446

Purpose

E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon

Depositor

Maciek R. Antoniewicz, Eleftherios Terry Papoutsakis

Article

Har et al Appl Microbiol Biotechnol. 2021 Jan;105(

Bacterial Resistance

Ampicillin and kanamycin

Available since

Aug. 18, 2021

Availability

Industry, Academic Institutions, and Nonprofits
MG1655 Z1 malE

Bacterial Strain

#65915

Purpose

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Bacterial Resistance

None

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
bSLS.114

Bacterial Strain

#191530

Purpose

Contains a knockout gene in the E. coli strain BL21-AI

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Species

Escherichia coli

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF6

Bacterial Strain

#62074

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
YM138

Bacterial Strain

#61953

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF10

Bacterial Strain

#62076

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. lysA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF1

Bacterial Strain

#61962

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF2

Bacterial Strain

#62070

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP

Bacterial Strain

#182776

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
MG1655 Z1 malE clpA

Bacterial Strain

#65918

Purpose

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, clpA KO

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Bacterial Resistance

None

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
MG1655 Z1 malE aat

Bacterial Strain

#65917

Purpose

E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, aat KO

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Bacterial Resistance

None

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only
RF13

Bacterial Strain

#62078

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF4

Bacterial Strain

#62072

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF5

Bacterial Strain

#62073

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB hisG genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
B-95.ΔAΔfabR

Bacterial Strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only
B-95.ΔA

Bacterial Strain

#197933

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne- lon-

Bacterial Strain

#182778

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Showing: 41 - 60 of 228 results

Results per page:

Sort results by:

Strain SIJ_488

C321.ΔA.exp

T-B18

MG1655 Z1 malE

bSLS.114

RF11

RF6

YM138

RF10

RF1

RF2

C321.∆A T7RNAP

MG1655 Z1 malE clpA

MG1655 Z1 malE aat

RF13

RF4

RF5

B-95.ΔAΔfabR

B-95.ΔA

C321.∆A T7RNAP ompT- rne- lon-

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