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Plasmid#98840PurposeProduces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)DepositorInsertsReach-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mGFP-10-sREACh-N3
Plasmid#21947DepositorInsertmGFP-10-sREACh
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-sREACh-Actin
Plasmid#21949DepositorInsertbetaActin
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
Gq-sensor[mTq2-sREACh]
Plasmid#98835PurposeA FRET sensor for Gq activation, encoded on a single plasmid, for FLIMDepositorInsertmTq2-sREACh
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-RIIα-sREACh
Plasmid#168489PurposeMammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.DepositorInsertPKA regulatory subunit IIα C-terminally tagged by sREACh
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-PKA-RIIβ-sREACh
Plasmid#114113PurposeMammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.DepositorInsertPKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
negEKARet-cyto
Plasmid#114734PurposeNegative control of EKARet-cytoDepositorInsertNegative Control of EKARet-cyto
UseTagsEGFP and sREAChetExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
negAKARet-cyto
Plasmid#114733PurposeNegative control of AKARet-cytoDepositorInsertNegative Control of AKARet-cyto
UseTagsEGFP and sREAChetExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EKARet-nuc
Plasmid#96896PurposeFLIM sensor for ERK activation in nucleusDepositorInsertFLIM Sensor for ERK Activity in Nucleus
UseTagsEGFP and sREAChetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
EKARet-cyto
Plasmid#96895PurposeFLIM sensor for ERK activation in cytosolDepositorInsertFLIM Sensor for ERK Activity in Cytosol
UseTagsEGFP and sREAChetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
AKARet-cyto
Plasmid#96897PurposeFLIM sensor for PKA activation in cytosolDepositorInsertFLIM Sensor for PKA Activity in Cytosol
UseTagsEGFP and sREAChetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only