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Plasmid#228409PurposeExpression of NIT-9 in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
C321.ΔA
Bacterial Strain#48998PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)DepositorBacterial ResistanceAmpicillinSpeciesEscherichia coliAvailable sinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_without_linker
Plasmid#228411PurposeExpression of NIT-9 without linkage region in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationdeletion of the linkage regionPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9fl_reverse
Plasmid#228410PurposeExpression of NIT-9 with reversed domains in N. crassa at the his-3 locusDepositorInsertNIT-9 domains reversed
UseProtein expression in n. crassaTagsExpressionMutationdomains reorientedPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9E
Plasmid#228419PurposeExpression of NIT-9E domain in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of the N terminal G domain and linkage…PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9G
Plasmid#228420PurposeExpression of NIT-9G domain in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of the C terminal E domain and linkage…PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_A184-G203
Plasmid#228421PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of A184-G203PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_G204-G223
Plasmid#228422PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of G204-G223PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_E224-G243
Plasmid#228423PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of E224-G243PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9_K264-P282
Plasmid#228425PurposeExpression of NIT-9 wit a 20 aa truncation in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of K264-P282PromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9G_linker
Plasmid#228413PurposeExpression of NIT-9G with linkage region at the C terminus in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of the C terminal E domainPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCCG_linker_nit9E
Plasmid#228412PurposeExpression of NIT-9E with linkage region at the N terminus in N. crassa at the his-3 locusDepositorInsertNIT-9
UseProtein expression in n. crassaTagsExpressionMutationtruncation of the N terminal G domainPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT_H1-S1
Plasmid#218400Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in first intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_S1-H1
Plasmid#218392Purpose3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
WT_S7-H1
Plasmid#218393Purpose3-exon minigene, with SRSF7 motif-rich middle exon, and hnRNPA1 motif-rich region in second intronDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-N
Plasmid#218398Purpose3-exon minigene, with SRSF1 motif-rich middle exon, neutral region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_N-H1
Plasmid#218397Purpose3-exon minigene, with neutral middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCG_nit9fl_CNX1_linker
Plasmid#228414PurposeExpression of NIT-9 with CNX1 linkage region in N. crassa at the his-3 locusDepositorInsertNIT-9 (AT5G61790 Mustard Weed)
UseProtein expression in n. crassaTagsExpressionMutationremoval of the linkage region and insertion of th…PromoterAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1
Plasmid#218394Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S7-H1
Plasmid#218396Purpose3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only