-
-
-
Grp1-PH pGEX2TK
Plasmid#71379Purposebacterial expression of PH domain for phosphoinositide binding assayDepositorInsertGrp1-PH (Cyth3 Mouse)
UseTagsGSTExpressionBacterialMutationPleckstrin homology (PH) domain; aa 264-391PromotertacAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Grp1-PH pEGFP-C1
Plasmid#71378PurposeFor analysis of PH domain localization in mammalian cellsDepositorInsertGrp1-PH (Cyth3 Mouse)
UseTagsEGFPExpressionMammalianMutationPleckstrin homology (PH) domain; aa 247-399PromoterCMVAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
GRPR-Tango
Plasmid#66394PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGRPR (GRPR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Grp1-PH pET11a
Plasmid#71377PurposePhosphoinositide BindingDepositorInsertGrp1-PH (Cyth3 Mouse)
UseTagsExpressionBacterialMutationPleckstrin homology (PH) domain; aa 264-391PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGrpE-Wasabi
Plasmid#86111PurposemWasabi reporter gene driven by GrpE promoterDepositorInsertmWasabi
UseTags6xHisExpressionBacterialMutationPromoterpGrpEAvailable sinceFeb. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
-
GRPR-DuET
Plasmid#213306PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRPR (GRPR Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK1520 sgRPS3
Plasmid#211526PurposeFacilitate knock-in of RPS3-Keima fusion proteinDepositorInsertsgRPS3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtNodGRP32:T35s
Plasmid#164756PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr5g084040.1
UseTagsTerminator 35SExpressionPlantMutationPromoterLotus japonicus UbiquitinAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GRP78/BiP
Plasmid#32701DepositorInsertGRP78/BiP (HSPA5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn grpLight1.2
Plasmid#175174Purposegenetically encoded GRP sensor grpLight1.2DepositorInserthSyn-driven grpLight1.2
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
R777-E245 Hs.RASGRP2
Plasmid#70529PurposeGateway ORF clone of human RASGRP2 [NM_153819.1] with stop codon (for native or N-terminal fusions)DepositorInsertRASGRP2 (RASGRP2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E243 Hs.RASGRP1
Plasmid#70527PurposeGateway ORF clone of human RASGRP1 [NM_005739.3] with stop codon (for native or N-terminal fusions)DepositorInsertRASGRP1 (RASGRP1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E249 Hs.RASGRP4
Plasmid#70533PurposeGateway ORF clone of human RASGRP4 [NM_001146202.1] with stop codon (for native or N-terminal fusions)DepositorInsertRASGRP4 (RASGRP4 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E247 Hs.RASGRP3
Plasmid#70531PurposeGateway ORF clone of human RASGRP3 [NM_001139488.1] with stop codon (for native or N-terminal fusions)DepositorInsertRASGRP3 (RASGRP3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
iRFP-PAmCherry1-PH_GRP1
Plasmid#92168PurposePI(3,4,5)P3 single molecule fluorescent reporterDepositorInsertiRFP-PAmCherry1-PHgrp1
UseTagsiRFP-PAmCherry1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only
