Addgene

We narrowed to 999 results for: gibson assembly

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pFloat.assemble(AB).LC_v1

Plasmid

#133083

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

Depositor

David Sabatini, Kacper Rogala

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFloat.assemble(AB).HC_v1

Plasmid

#133084

Purpose

Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

Depositor

David Sabatini, Kacper Rogala

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Destination vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDL1

Plasmid

#182263

Purpose

Gibson cloning vector for synthesis of single and double stranded RNA

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Type

Empty backbone

Use

Gibson cloning vector for synthesis of single and…

Tags

Expression

Mutation

Promoter

T3 and SP6 for sense and antisense riboprobe synt…

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-T

Plasmid

#87522

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-A

Plasmid

#87523

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-C

Plasmid

#87524

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-G

Plasmid

#87525

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAW2-Smed-Slit-1

Plasmid

#182264

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Slit-1

Depositor

Daniel Lobo

Article

Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do

Insert

Smed-Slit-1

Use

Gibson cloning vector for synthesis of single and…

Tags

Expression

Mutation

Promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'omega

Plasmid

#223870

Purpose

4G-cloning donor for 3'-Gibson overhang, omega-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'omega sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'alpha

Plasmid

#223863

Purpose

4G-cloning donor for 5'-Gibson overhang, alpha-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'alpha sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'gamma

Plasmid

#223865

Purpose

4G-cloning donor for 5'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'gamma sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'beta

Plasmid

#223864

Purpose

4G-cloning donor for 5'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'beta sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_5'delta

Plasmid

#223866

Purpose

4G-cloning donor for 5'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

5'delta sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'beta

Plasmid

#223867

Purpose

4G-cloning donor for 3'-Gibson overhang, beta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'beta sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'gamma

Plasmid

#223868

Purpose

4G-cloning donor for 3'-Gibson overhang, gamma-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'gamma sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Gib)_3'delta

Plasmid

#223869

Purpose

4G-cloning donor for 3'-Gibson overhang, delta-sequence

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3'delta sequence and spacer

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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biGBac Kit

Plasmid Kit

#1000000088

Purpose

Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.

Depositor

Jan-Michael Peters

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
pJRA99

Plasmid

#209334

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of untagged protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJRA-GW

Plasmid

#209333

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS110

Plasmid

#209331

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal StayGold fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge