Addgene

We narrowed to 48 results for: cjcas9

Showing: 1 - 20 of 48 results

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pET-CjCas9

Plasmid

#89754

Purpose

Expression of CjCas9 with His tag in E.coli

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

CRISPR

Tags

6XHis, HA, and SV40 NLS

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-CjCAS9-pA

Plasmid

#131543

Purpose

Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:

Insert

CjCas9

Use

CRISPR

Tags

FLAG-SV40NLS, SV40NLS, and polyA

Expression

Mammalian

Mutation

Human codon-optimized

Promoter

CAG, T3

Availability

Academic Institutions and Nonprofits only

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LentiGuide_CjCas9-Puro

Plasmid

#202564

Purpose

hU6-BsmBI-gRNA-Puro

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

CjCas9 sgRNA cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-CjCas9

Plasmid

#89752

Purpose

Expression of CjCas9 in mammalian cells

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cas9

Use

CRISPR

Tags

SV40-HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pX551-miniCMV-CjCas9

Plasmid

#107033

Purpose

AAV plasmid expressing CjCas9 under control of miniCMV promoter

Depositor

Alex Hewitt

Insert

CjCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pX551-CMV-CjCas9

Plasmid

#107035

Purpose

AAV plasmid expressing CjCas9 under control of CMV promoter

Depositor

Alex Hewitt

Insert

CjCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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U6-CjCas9-MS2gRNA-Backbone

Plasmid

#210711

Purpose

This plasmid express MS2 loop containing Cj Cas9 gRNA

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MS2 loop containing Cj Cas9 gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-ABE8e_gRNA

Plasmid

#202559

Purpose

P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-eAID_gRNA

Plasmid

#202562

Purpose

P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-TadCDd_gRNA

Plasmid

#202563

Purpose

P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-enCjCas9-BlastR

Plasmid

#220978

Purpose

Expresses human codon-optimized enCjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

enCjCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-CjCas9-BlastR

Plasmid

#220979

Purpose

Expresses human codon-optimized CjCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

CjCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-BlastR

Plasmid

#194059

Purpose

Mammalian expression of evoCjCas9 nuclease

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CMV-VPR-S-L1-dCjCas9 MiniCAFE

Plasmid

#169910

Purpose

Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

MiniCAFE

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D8A, HNH-truncation (Δ495–609 aa) in CjCas9

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

Plasmid

#169912

Purpose

Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

10XGCN4, 2xSV40NLS, and SV40NLS-HA

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-VPR-L1-CjCas9 V-D

Plasmid

#169913

Purpose

Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-VPR-WT CjCas9 V-WT

Plasmid

#169914

Purpose

Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

CjCas9

Use

CRISPR

Tags

2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-evoCjCas9-ABE8e_gRNA

Plasmid

#202561

Purpose

hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CjCas9-eGFP-HIF1a

Plasmid

#137929

Purpose

Expression of cjCas9 with Hif1a targeting sgRNA

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

AAV and CRISPR

Tags

SV40NLS-HA-T2A-EGFP

Expression

Mutation

Promoter

EF-1alpha

Availability

Academic Institutions and Nonprofits only

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