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pAAV-Ef1a-DIO-PPO-Venus

Plasmid

#139505

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV8 and AAV9

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-DIO-PPO-Venus

Plasmid

#139504

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV5

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-OMOR-eYFP

Plasmid

#66847

Purpose

Expresses Opto-MOR in a Cre dependent manner

Depositor

Michael Bruchas

Article

Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

Insert

Opto Mu opioid receptor

Use

AAV and Cre/Lox

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Use

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PPO-mScarlet

Plasmid

#139503

Purpose

Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Use

Tags

mScarlet following Gly-Gly-Ser linker

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Use

Tags

N-terminal mScarlet tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Use

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-optoB2-LYY-YFP

Plasmid

#75271

Purpose

G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

G-protein independent version of optoB2

Use

Tags

eYFP

Expression

Mammalian

Mutation

point mutations at L72F,Y136G,Y224A

Promoter

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-PPO-Venus

Plasmid

#139505

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV8 and AAV9

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-DIO-PPO-Venus

Plasmid

#139504

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has Service

AAV5

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-OMOR-eYFP

Plasmid

#66847

Purpose

Expresses Opto-MOR in a Cre dependent manner

Depositor

Michael Bruchas

Article

Siuda et al Neuron. 2015 May 20;86(4):923-35. doi: 1

Insert

Opto Mu opioid receptor

Use

AAV and Cre/Lox

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PPO-Venus

Plasmid

#139502

Purpose

Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Use

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-PPO-mScarlet

Plasmid

#139503

Purpose

Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Use

Tags

mScarlet following Gly-Gly-Ser linker

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mScarlet-gamma9

Plasmid

#140097

Purpose

Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

GNGT2

Use

Tags

N-terminal mScarlet tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Use

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-optoB2-LYY-YFP

Plasmid

#75271

Purpose

G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

G-protein independent version of optoB2

Use

Tags

eYFP

Expression

Mammalian

Mutation

point mutations at L72F,Y136G,Y224A

Promoter

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge