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Plasmid#184566PurposeInducible expression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-kan
Plasmid#184836PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA4
Plasmid#101043PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA4
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA2
Plasmid#101042PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA2
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcrVIB1-cloDF-amp
Plasmid#184843PurposeExpression of anti-CRISPR protein AcrVIB1 in bacteria.DepositorInsertanti-CRISPR protein inhibiting Cas13b nucleases
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS540-pCSDest2-AcrIIC4Hpa
Plasmid#113434PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS542-pCSDest2-AcrIIC5Smu
Plasmid#113435PurposeExpresses type II-C anti-CRISPR protein from S. muelleri in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS663-pMCSG7-AcrIIC4Hpa
Plasmid#113440PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from H. parainfluenzae in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
UseTags6xHisExpressionBacterialMutationPromoterlacIAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS664-pMCSG7-AcrIIC5Smu
Plasmid#113441PurposeExpresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cellsDepositorInsertType II-C anti-CRISPR with N-terminal 6xHis and TEV site
UseTags6xHisExpressionBacterialMutationPromoterlacIAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X
Plasmid#128112PurposeAcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9DepositorInsertAcrX (AcrIIC1 N3F/D15Q/A48I)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS
Plasmid#113436PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cellsDepositorInserttype II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS
Plasmid#113437PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES
Plasmid#113438PurposeExpresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS593-pCSDest2-AcrIIC5Smu-BFPv2-IRES
Plasmid#113439PurposeExpresses type II-C anti-CRISPR protein from S. muelleri and a BFP from IRES in mammalian cellsDepositorInsertsType II-C anti-CRISPR
mTagBFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X* (AcrIIC1X-mCherry chimera)
Plasmid#128114PurposeExpresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9DepositorInsertAcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1-mCherry chimera #10
Plasmid#128113PurposeExpresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9DepositorInsertAcrIIC1-mCherry chimera #10
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS433-pCSDest2-AcrIIC1Nme
Plasmid#85679PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC1Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC1Nme
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS436-pCSDest2-AcrIIC2Nme
Plasmid#85712PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC2Nme
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS443-pCSDest2-AcrIIC3Nme
Plasmid#85713PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC3Nme from Neisseria meningitidisDepositorInsertType II-C anti-CRISPR AcrIIC3Nme
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS430-pCSDest2-AcrIIC1Boe
Plasmid#85678PurposeMammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodisDepositorInsertType II-C anti-CRISPR AcrIIC1Boe
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only