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Plasmid#140536PurposeAAVS1 CMV-OsTIR1(F74G)DepositorInsertCMV-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 T2 CRIPR in pX330
Plasmid#72833PurposeCRISPR/Cas to target the AAVS1 locus in human cellsDepositorInsertCas9 and gRNA for targeting the AAVS1 locus in human cells
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Hygro)
Plasmid#140646PurposeCTCF tagging with mAID-cloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK286 (mAID-NeoR)
Plasmid#72824PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Neo
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Tet-OsTIR1(F74G)-V5
Plasmid#158664PurposeTet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locusDepositorInsertTet-OsTIR1(F74G) AAVS1
UseCRISPRTagsExpressionMammalianMutationF74GPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)
Plasmid#140659PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NlucDepositorInsertOsTIR1(F74G) mAID-EGFP-Nluc TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK262 (RAD21-mAC Neo)
Plasmid#140538PurposeRAD21 tagging with mAID-CloverDepositorInsertRAD21 (RAD21 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
mAID-BRD4 donor
Plasmid#140650PurposeBRD4 tagging with mAIDDepositorInsertBRD4 (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRD4-N CRISPR
Plasmid#140651PurposeBRD4 tagging CRISPRDepositorInsertBRD4 (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMC2-mAC donor (Hygro)
Plasmid#140648PurposeSMC2 tagging with mAID-CloverDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK434 (mAID-mClover-P2A-Hygro)
Plasmid#168229PurposeC-terminal tagging with mAID-mCloverDepositorInsertmAID-mClover-P2A-Hygro
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)
Plasmid#140533PurposeOsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAY5 (mAID-EGFP-NLS piggyBac)
Plasmid#140532PurposemAID-EGFP-NLS piggyBacDepositorInsertmAID-EGFP-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK349 (BSD-P2A-mAID-mCherry)
Plasmid#121183PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-mCherry
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK444 (EF1-OsTIR1(F74G) Puro)
Plasmid#214374PurposeEF1-OsTIR1(F74G) (PiggyBac)DepositorInsertEF1-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterEF1 promoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-OsTIR1(F74G)
Plasmid#140730PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NESDepositorInsertOsTIR1(F74G)-P2A-mAID-EGFP-NES
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)
Plasmid#140535PurposeOsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Plasmid#121184PurposeOsTIR1 introductionDepositorInsertCMV-OsTIR1-loxP-PURO-loxP
UseCRISPRTagsExpressionMutationOsTIR1 codon optimized for human cellsPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK395 (AAVS1 CMV-OsTIR1(F74A))
Plasmid#159992PurposeExpression of OsTIR1(F74A) in human cellsDepositorInsertCMV-OsTIR1(F74A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only