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Plasmid#27388PurposeBurkholderia allelic exchange suicide vector pMo130, ref EU862243DepositorTypeEmpty backboneUseBurkholderia allelic exchange suicide vector pmo1…TagsExpressionMutationPromoterAvailable sinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only
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pMo168
Plasmid#27389DepositorInsertpMo168
UseTagsExpressionBacterialMutationA replicative vector for gene expression in Burkh…PromoterAvailable sinceMarch 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BpGmhA
Plasmid#125589PurposeOverexpression plasmid for production of GmhA from Burkholderia pseudomalleiDepositorInsertBpGmhA (gmhA Burkholderia pseudomallei)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Tp-SceI-pheS
Plasmid#111608PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Km-SceI-pheS
Plasmid#111610PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b/BOA
Plasmid#82041PurposeFor E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)DepositorInsertBurkholderia oklahomensis agglutinin
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Gm-SceI-pheS
Plasmid#111609PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD
Plasmid#32055PurposeVector optimized for inducible gene expression in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPbadAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCYP05
Plasmid#212838PurposeExpresses the Burkholderia multivorans LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-CM
Plasmid#203588PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2
Plasmid#113634PurposeAn expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepaciaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGPI-SceI
Plasmid#32060PurposeConstruction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of BurkholderiaDepositorTypeEmpty backboneUseMutagenesis plasmidTagsExpressionMutationPromoterAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLS7
Plasmid#32056PurposeVector optimized for constitutive gene expression in Burkholderia genus and related bacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPs7 promoter, originated from Paraburkholderia xe…Available sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-TP
Plasmid#203589PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-KM
Plasmid#203587PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9
Plasmid#129391PurposeDerived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.DepositorInsertBurkholderia cenocepacia codon-optimized dCas9
UseCRISPRTagsExpressionBacterialMutationEntire dCas9 codon optimized for the GC-rich B. c…PromoterPrhaBADAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZLrhaB2plus
Plasmid#211787PurposepSCrhaB2plus with modifications of resistance cassette to CmRDepositorInsertCmR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BpWcbI
Plasmid#125591PurposeOverexpression plasmid for production of WcbI from Burkholderia pseudomalleiDepositorInsertBpWcbI (wcbI Burkholderia pseudomallei)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp-fugfp
Plasmid#211790PurposepDonorTp with addition of fugfp gene from pUS252 outside of the FRT sitesDepositorInsertfuGFP
UseTagsExpressionMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only