1. pU6-(BbsI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #64324

   Purpose

   Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. NSD1 scFv [N312/10]

   Plasmid

   #206779

   Purpose

   Mammalian Expression of NSD1 scFV. Derived from hybridoma N312/10 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   NSD1 (Homo sapiens) recombinant scFV (NSD1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CL20_mEGFP_NUP98_NSD1

   Plasmid

   #205873

   Purpose

   Express mEGFP-tagged fusion protein, NUP98_NSD1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NUP98_NSD1 (NUP98, NSD1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CL20_mEGFP_NSD1_ZNF346

   Plasmid

   #205870

   Purpose

   Express mEGFP-tagged fusion protein, NSD1_ZNF346 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   NSD1_ZNF346 (NSD1, ZNF346 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Anti-NSD1 [N312/10]

   Plasmid

   #190311

   Purpose

   Mammalian Expression Plasmid of anti-NSD1 (Human). Derived from hybridoma N312/10.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-NSD1 (Homo sapiens) recombinant Mouse monoclonal antibody (NSD1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

   Plasmid

   #117070

   Purpose

   Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection

   Depositor

   Kim Failor

   Inserts

   Cas9
   SacB
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Intron-Tagging-pX330-Cas9-Blast

   Plasmid

   #159741

   Purpose

   Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Intron-Tagging-pX330-Cas9-mCherry

   Plasmid

   #159742

   Purpose

   Expresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B

   Plasmid

   #64221

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-mCherry-P2A-E1B

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU6-(BsaI)_CBh-Cas9-T2A-mCherry

   Plasmid

   #135012

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptide

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   3x Flag, 3xFLAG (N terminal on insert), NLS, and …

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid

    #64222

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEmerald MAP1LC-C

    Plasmid

    #54151

    Purpose

    Localization: Microtubules, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    MAP1LC (MAP1LC3B2 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry

    Plasmid

    #135014

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptide

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3x Flag, 3xFLAG (N terminal on insert), NLS, and …

    Expression

    Mammalian

    Mutation

    126aa domain from HSV-1 UL12 fused to the C-termi…

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

    Plasmid

    #135013

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Tags

    3x Flag, 3xFLAG (N terminal on insert), NLS, and …

    Expression

    Mammalian

    Mutation

    126aa domain from HSV-1 UL12 fused to the N-termi…

    Promoter

    CBh

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223 LC3B2 WT

    Plasmid

    #123075

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223 LC3B2 G120

    Plasmid

    #123077

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 G120

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR223 LC3B2 G120A

    Plasmid

    #123076

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 G120A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Glycine 120 to Alanine

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223 LC3B2 no-stop

    Plasmid

    #123207

    Purpose

    Gateway entry clone encoding human MAP1LC3B2 lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Gateway entry vector / entry clone

    Tags

    Expression

    Mutation

    Stop codon removed

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-CMV 3xFLAG-LC3B2-GFP

    Plasmid

    #123109

    Purpose

    Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Use

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSICO-CPSF6-358-eGFP

    Plasmid

    #110693

    Purpose

    Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

    Depositor

    Zandrea Ambrose

    Article

    Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

    Insert

    CPSF6-eGFP (CPSF6 )
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Truncation of CPSF6 isoform 2 at amino acid 358

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge