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Plasmid#173121PurposeExpresses TEVp(WT) and reporter receiver construct in DIPGDepositorInsertArrestin-TEVp(WT)-V5; UAS-mCherry
UseTagsExpressionMammalianMutationPromoterUbCAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV CCR6-eLOV-TEVcs-Gal4
Plasmid#173113PurposeExpresses CCR6 receiver construct in mammalian cellsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFPxm163
Plasmid#193480PurposeTurn-off fluorescent sensor for chlorideDepositorInsertGFPxm163
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8133 MSCV GFP Neo C-HA
Plasmid#184522PurposeExpression of GFPDepositorInsertGFP
UseRetroviralTagsHAExpressionMutationPromoterMSCV LTRAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm2_A
Plasmid#145887PurposeInsect Expression of DmLSm2DepositorInsertDmLSm2 (CG10418 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm5_A
Plasmid#145890PurposeInsect Expression of DmLsm5DepositorInsertDmLsm5 (CG6610 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgRNA HBB
Plasmid#128120PurposeCo-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA HBB + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA RUNX
Plasmid#128122PurposeCo-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA RUNX + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA HEK
Plasmid#128123PurposeCo-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)DepositorInsertsgRNA HEK + GFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRSV/U6Available sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFP-tTA-ER-C3
Plasmid#134569PurposeExpresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion proteinDepositorInserttTA-ER
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TET1-746-951(pL72)
Plasmid#124406PurposeTET1-746-951, containing SID, used for Co-IP.DepositorInsertFLAG-TET1-746-951 (TET1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET30a-RAMA
Plasmid#112226PurposeExpresses RAMA domain from murine MPNDDepositorInsertMPND (Mpnd Mouse)
UseTagsFlag and HIs S-tagExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-BBa_B0012
Plasmid#72997PurposeBBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)DepositorInsertBba_B0012 terminator
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-BBa_B0015
Plasmid#72998PurposeBBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)DepositorInsertBBa_B0015 terminator
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2_2b
Plasmid#73011PurposeSecondary module (BpiI) site in pTU2b backboneDepositorInsertSecondary module + Golden Gate pTU2b BsmBI destination site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-eCFP
Plasmid#72961PurposeLevel 0 Entry vector" ORF - eCFP"DepositorInsertEnhanced cyan fluorescent protein (eCFP)
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-ORF-mCherry
Plasmid#72962PurposeLevel 0 Entry vector" ORF - mCherry "DepositorInsertmCherry fluorescent protein
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM901
Plasmid#72966PurposeLevel 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM901 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-SJM902
Plasmid#72967PurposeLevel 0 - SJM902 promoter (5' CTAT/3' GTAC fusion)DepositorInsertSJM902 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only