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Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
UseTags6His-TEVExpressionBacterialMutationR49APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
eGFP-Silicatein-pET28
Plasmid#198012PurposeC-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purificationDepositorInserteGFP-silicatein
UseTagshexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
883 pET15b PTEN
Plasmid#10936DepositorInsertPTEN (PTEN Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-2HMG-mCherry
Plasmid#112739PurposeExpress 2HMG-mCherry in E. coliDepositorInsert2HMG-mCherry
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmBelle_B
Plasmid#145963PurposeBacterial Expression of DmBelleDepositorInsertDmBelle (bel Fly)
UseTagsExpressionBacterialMutationdel 155-157, T530A and I685VPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2H5
Plasmid#71715PurposeMembrane scaffold protein (MSP), helix 4/2 + helix5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
CBP-HAT-pET
Plasmid#21091DepositorInsertCBP (Crebbp Mouse)
UseTagsHis and T7 tagExpressionBacterialMutationHAT domain aa 1171-1621PromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-Q11
Plasmid#185781PurposeEncodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-Q11
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
5787 pET28aSX104B/ pES42
Plasmid#1229DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsHisExpressionBacterialMutationnonePromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del3 pET28a
Plasmid#122117PurposeExpression Brg1 amino acids 836-1446 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28b-GBP-MTn
Plasmid#154363PurposeBacterial expression of MTnDepositorInsertMTn
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-baPrs-hdNadV
Plasmid#91950PurposepET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyiDepositorInsertsPutative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases
UseTagsExpressionBacterialMutationchanged Leucine 135 to IsoleucinePromoterT7Available sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2J1 1-283
Plasmid#185348PurposeIn vitro transcription and translation in bacterial systemDepositorInsertUBE2J1 (UBE2J1 Human)
UseTagsExpressionBacterialMutationCytoplasmic domain only, aa 1-283PromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Tre
Plasmid#123129PurposeBacterial expression plasmid for Tre with an N-terminal His-tag.DepositorInsertTre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30-hDlg PDZ2
Plasmid#21692DepositorInserthDlg/SAP97 PDZ2 domain (DLG1 Human)
UseTagsHis6ExpressionBacterialMutationhuman hDlg/SAP97 PDZ2 domain (residues 318-405)PromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTags(His)6-ThioredoxinExpressionBacterialMutationPromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-STE2
Plasmid#133430PurposeYeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionBacterialMutationBoth native Cys (C59S/C252A) have been mutated. S…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-CXCR4
Plasmid#133431PurposeHuman CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertCXCR4 (CXCR4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only