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Showing: 1961 - 1980 of 11984 results

  4. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  8. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Use
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3-FRB-VMAc-CGFP

    Plasmid
    #70226
    Purpose
    Expresses C-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Use
    Tags
    FKBP-rapamycin binding (FRB) domain of the mammal…
    Expression
    Mammalian
    Mutation
    Changed Ile 129 to Cys, Deleted amino acids 1-128
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pZA-GFP-R5

    Plasmid
    #106256
    Purpose
    Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide
    Depositor
    Urartu Seker
    Article
    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi
    Insert
    Green fluorescent protein
    Use
    Tags
    Added silaffin R5 peptide to C terminus of GFP af…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    106256-plasmid-map-sequence-id-232729

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  11. NODALvar-CAG-AAVS1

    Plasmid
    #115262
    Purpose
    For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells
    Depositor
    Lynne Postovit
    Article
    Findlay et al (2018) bioRxiv 276170
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    115262-plasmid-map-sequence-id-222505

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  14. NSarQ27G-VMAn-FKBP

    Plasmid
    #70230
    Purpose
    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Use
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pZA-mCherry-R5

    Plasmid
    #106258
    Purpose
    Expresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetO
    Depositor
    Urartu Seker
    Article
    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi
    Insert
    mCherry
    Use
    Tags
    Added silaffin R5 peptide to C terminus of mCherr…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1961 - 1980 of 11984 results