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Plasmid#158980PurposeConstitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.DepositorInsertmruby-iATPSNfR1.0
UseTagsmruby3ExpressionBacterialMutationcodon optimized for e. coli from Addgene#102557PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV-EF1s HA.NLS.Sce(opt)
Plasmid#31479DepositorInsertSFFV/Ef1s HA NLS I-SceI
UseLentiviralTagsHA and NLSExpressionMutation"opt" indicates the coding sequence has…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_VSBV_G
Plasmid#207312PurposeMammalian expression of Variegated Squirrel Bornavirus G proteinDepositorInsertVSBV_G
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-PC
Plasmid#92125PurposeExpression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reeseiDepositorInsertCas9
UseCRISPR; Trichoderma reesei expressionTagsExpressionMutationPromoterPpdcAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pALPS vpu
Plasmid#101330PurposeEncodes codon optimized NL4-3 vpuDepositorInsertNL4-3 vpu
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LuxI
Plasmid#193626PurposeConstitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.DepositorInsertLuxI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_YFV_PrME
Plasmid#207315PurposeMammalian expression of Yellow fever virus PrME proteinDepositorInsertYFV_PrME (POLY Yellow fever virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_BoDV1_G
Plasmid#207256PurposeMammalian expression of Borna disease virus 1 G proteinDepositorInsertBoDV1_G
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_BoDV2_G
Plasmid#207257PurposeMammalian expression of Borna disease virus 2 G proteinDepositorInsertBoDV2_G
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_BRV_GP
Plasmid#207258PurposeMammalian expression of Blue River virus GP proteinDepositorInsertBRV_GP
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pfMDH_WT
Plasmid#67721PurposeExpresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coliDepositorInsertMannitol 2-Dehydrogenase from Pseudomonas fluorescens
UseTags6x-His tagExpressionBacterialMutationPromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALPS vif
Plasmid#101328PurposeEncodes codon optimized NL4-3 vifDepositorInsertNL4-3 vif
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pALPS rev
Plasmid#101327PurposeEncodes codon optimized NL4-3 revDepositorInsertNL4-3 rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 Spike-d18
Plasmid#164583PurposeA mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.DepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2)
UseTagsExpressionMammalianMutationDeleted amino acids 1256-1273PromoterEF1aAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALPS vpr
Plasmid#101329PurposeEncodes codon optimized NL4-3 vprDepositorInsertNL4-3 vpr
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDD285
Plasmid#66826PurposemKate2^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmKate2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized mKate2ExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALPS tat
Plasmid#101326PurposeEncodes codon optimized NL4-3 tatDepositorInsertNL4-3 tat
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_dTK
Plasmid#133244PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertdrosophila tachykinin-fused codon-optimized GCaMP6s
UseTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL12-GCaMP6f-pHuji bb118
Plasmid#158981PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.DepositorInsertGCaMP6f-pHuji
UseTagsExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833 a…PromoterBba_J23118 (sigma 70)Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only