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Plasmid#168100PurposeHA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nBC2-OGA(544-706)
UseTagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras heavy chain-GSG-SpyTag003
Plasmid#216292PurposeExpresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras FabDepositorInsertTras heavy chain-GSG-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dL5-2xG4S-TMst
Plasmid#73206PurposeExpresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertkappa-myc-dL5-2XG4S-TM (MYC Human)
UseTagsThe FAP is fused with 2 copies of a G4S linker an…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag Cl-sensitive YFP hNKCC1 WT (NT13)
Plasmid#49060PurposeExpresses human NKCC1 with an N-terminal 3xFlag-YFP-(chloride-sensing) tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequenceDepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and YFP (chloride-sensitive)ExpressionMammalianMutationCl-sensitive YFP (EYFP/ V163S A206K) in hNKCC1 in…PromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 His-GFP-EWSR1-myc-His
Plasmid#46385Purposeexpresses EGFP tagged EWSR1 in mammalian cellsDepositorInsertEWSR1 (EWSR1 Human)
UseTagsEGFP, His, and mycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
(114) pcDNA3.1-GFP-Flag-JunB-EPEA
Plasmid#160743PurposeExpresses a GFP tagged JunB with Flag and EPEA tags.DepositorInsertGFP-Flag-JunB-EPEA (JUN Human)
UseTagsEPEA, Flag, and GFPExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3
Plasmid#73205PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)DepositorInsertNLS-myc-dL5-2XG4S-mCer3 (MYC Human, Synthetic)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
(112) pcDNA3.1-GFP-Flag-Nup62-EPEA
Plasmid#168101PurposeGFP and Flag tagged Nup62 with EPEA nanobody tagDepositorInsertGFP-Flag-Nup62-EPEA
UseTagsEPEA, Flag, and GFPExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)
Plasmid#86886PurposeN-terminal part of the split-BioIDDepositorInsertPP1g - FLAG - BirA (AA2-140)
UseTagsFLAG tagExpressionMammalianMutationBirA Amino Acids 2-140PromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3
Plasmid#73208PurposeExpresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)DepositorInsertCOXIV-COX8-dL5-2XG4S-mCer3 (COX8A Human, Synthetic)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TAT-1-101-FLAG basic mutant
Plasmid#35985DepositorInserttransactivating protein (tat )
UseTagsFlagExpressionMammalianMutationtat from HIV1, 101 aa; R to A mutations at aa 56,…PromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
(318) pcDNA3.1-myc-OGA(1-400)D174N
Plasmid#168096PurposeMyc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutationDepositorInsertmyc-OGA(1-400, D174N)
UseTagsmycExpressionMammalianMutationD174N catalytically inactive OGAPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFLAG TPP1 88-544 delta166-172
Plasmid#53551Purposemammalian expression of TPP1 full length with 166-172 replaced with "GSSG"DepositorInsertTPP1 (ACD Human)
UseTags3xFLAGExpressionMammalianMutationcontains aa 88-544, with aa 166-172 replaced wit…PromoterCMVAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD40L (lose of function mutant)-mgSrtA
Plasmid#125796Purposefor co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.DepositorInsertCD40L (lose of function mutant)-mgSrtA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1461R
Plasmid#163990PurposeMammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tagDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationE1461RPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR
Plasmid#187089PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-SF-iGluSnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hygro(+)-PSTCD-cGAS-delta DBD
Plasmid#102606PurposeMammalian expression of mutant cGAS with deletion of DNA binding domain fused to PSTCD tagDepositorInsertcGAS (CGAS Human)
UseTagsExpressionMammalianMutationdelta K173-I220 and delta H390-C405PromoterCMVAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR
Plasmid#187091PurposeGABA sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-iGABASnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR
Plasmid#187092PurposeGABA sensor for localization to membrane contatctDepositorArticleInsertPost-SPHERE-iGABASnFR
UseTagsiRFP670-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only