Addgene

We narrowed to 18,300 results for: flag

Showing: 1941 - 1960 of 18300 results

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pCDNA3 Flag Jnk2b1

Plasmid

#13756

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2b1 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pCS2+8 Flag2AUF1(p40)

Plasmid

#135945

Purpose

Expresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p42)

Plasmid

#135946

Purpose

Expresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-mEKLF

Plasmid

#67833

Purpose

Expression of FL-EKLF driven by SV40 promoter

Depositor

James Bieker

Article

Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

EKLF (Klf1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

SV40

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-Flag-Med26

Plasmid

#49250

Purpose

Mammalian expression of flag-tagged Med26

Depositor

Ronald Conaway, Joan Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)_No_FLAG_ATP1A1-G7

Plasmid

#173203

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAGHA-SCAF8

Plasmid

#122470

Purpose

Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF8 isoform C CRISPR resistant (SCAF8 Human)

Use

Tags

FLAGHA

Expression

Mammalian

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pKK-FLAG-TEV

Plasmid

#105768

Purpose

Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

FLAG-TEV

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC2-GPT2-3xFLAG

Plasmid

#163447

Purpose

lentiviral overexpression vector for human GPT2

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT2 (GPT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-dHeFSpCas9

Plasmid

#92116

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive HeFSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

Promoter

Cbh

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac mph1 flag

Plasmid

#1985

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

mPh1 (Phc1 Mouse)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pFRT/TO/SCAF4-FLAG

Plasmid

#122469

Purpose

Dox-inducible FLAG (C-terminal tag) SCAF4 expression

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 (SCAF4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV10-Flag-Ovtm

Plasmid

#51888

Purpose

expression vectore in mammalian cells

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mFbxl3 (Fbxl3 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

ovtm (I364T)

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
RCASBP(A)-3xFlag-mHoxd13wt

Plasmid

#145288

Purpose

Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

Hoxd13(wt) (Hoxd13 Mouse)

Use

Viral avian expression

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEBB COMMD6 Flag

Plasmid

#74898

Purpose

Express COMMD6 in mammalian cells

Depositor

Colin Duckett, Ezra Burstein

Article

Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

Insert

COMMD6 (COMMD6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF-1alpha promoter

Available since

June 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-flag-UTR

Plasmid

#183234

Purpose

Mammalian expression empty backbone

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-Flag

Plasmid

#67164

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

FLag

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac flag Mel18

Plasmid

#1968

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Mel18 (Pcgf2 Mouse)

Use

Tags

flag

Expression

Insect

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA6-N-3XFLAG-Gp78

Plasmid

#123590

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

autocrine motility factor receptor (Amfr Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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