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Plasmid#174333Purposeencodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsmCherry tagExpressionMammalianMutationNKD2132DFD134>AFA (substitutions of D132 and D…PromoterCMVAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-GFP
Plasmid#129092PurposeKI EGFP with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertEGFP and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag‐hTim1
Plasmid#49207Purposeexpresses Flag-tagged human Tim1DepositorInsertTim1 (HAVCR1 Human)
UseTagsFLAG and signal sequenceExpressionMammalianMutationTim1 lacks its own signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmReNL
Plasmid#182970PurposeStably express PalmReNL in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmReNL
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-CreER
Plasmid#129093PurposeKI CreER with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertCreERT2 and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmNanoLuc
Plasmid#182969PurposeStably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmNanoLuc
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PRMT6
Plasmid#164697PurposeExpression of PRMT6 with an N-terminal FLAG tagDepositorInsertPRMT6 (PRMT6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-hCRACR2A-isoform-a
Plasmid#79593PurposeTransient expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3
Plasmid#49208Purposeexpresses Flag tagged mouse Tim3DepositorInsertTim3 (Havcr2 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationno Tim3 signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim1 (Bl/6)
Plasmid#49205Purposeexpresses Flag tagged mouse Tim1DepositorInsertTim1 (BL/6 allele) (Havcr1 Mouse)
UseTagsFLAG and signal sequenceExpressionMammalianMutationTim1 lacks its own signal sequencePromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Farnesyl-5
Plasmid#57613PurposeLocalization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertFarnesyl (HRAS Human)
UseTagstdEosExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBY011-AMN1ko
Plasmid#183099PurposeS. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.DepositorInsertAMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
UseTagsKanMXExpressionBacterial and YeastMutationonly includes external sequences (504 bp each) as…PromoterURA3, TEF1Available sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmGamillus
Plasmid#182971PurposeStably express PalmGamillus in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmGamillus
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHL708
Bacterial Strain#98417PurposeBacterial Strain with Genotype: MC4100 ∆clpPXDepositorBacterial ResistanceStreptomycinAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
FW102 OL2-62
Bacterial Strain#53735PurposeBacterial-two hybrid reporter strainDepositorBacterial ResistanceKanamycin and streptomycinSpeciesEscherichia coliAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HR180-LGR5-iCT
Plasmid#129094PurposeKI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locusDepositorInsertiCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
UseHomologous recombination donor plasmidTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR-HA-IKKbeta
Plasmid#15470DepositorInsertIkB kinase beta (Ikbkb Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKbeta
Plasmid#15465DepositorInsertIkB kinase beta (Ikbkb Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP
Plasmid#61527PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP(TA)
Plasmid#61528PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TA mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TA mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only