-
Plasmid#50872PurposeExpresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationL671C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)
Plasmid#50871PurposeExpresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationE670C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFUS_A30B
Plasmid#31030DepositorInsertLacZ + BsaI restriction sites
UseTagsExpressionMutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mTagBFP2-2A
Plasmid#102602PurposeControl lentivector. Coexpression of mTagBFP and gene of interest from CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTet-IRES-EGFP
Plasmid#64238PurposeLentiviral, doxycycline-inducible system to express transgene of interest and EGFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUltra
Plasmid#24129Purpose3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interestDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro
Plasmid#1764PurposeRetroviral vector for cloning and expressing your gene of interest. Puromycin selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
myc-BioID2-MCS
Plasmid#74223PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsMyc and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro
Plasmid#1765PurposeRetroviral vector for cloning and expressing your gene of interest. Hygromycin selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minPro
Plasmid#211517PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minPro-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minProAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mycBioID2-pBABE-puro
Plasmid#80900PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagshumanized A. aeolicus BioID2 with R40G and mycExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-C
Plasmid#118372PurposeGateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo
Plasmid#1767PurposeRetroviral vector for cloning and expressing your gene of interest. Neomycin selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBABE-zeo (pBABE-bleo)
Plasmid#1766PurposeRetroviral vector for cloning and expressing your gene of interest. Zeocin selection.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-CD4-sTb(C)-M13-GFP
Plasmid#219784PurposesTb(C) half of CaST for neuron expressionDepositorInsertCD4-sTb(C)-M13-GFP
UseTagsHAExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-N
Plasmid#118373PurposeGateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only