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Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pLenti spCas9 T2A mNeonGreen P2A puro
Plasmid#122183PurposeThe plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-LC3B G120
Plasmid#123241PurposeExpression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acids 121-125. Stop codon after G12…PromoterPGKAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV/TO GFP-Zeo DEST (719-1)
Plasmid#17431Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-ZeoDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A TagRFP-T P2A puro
Plasmid#122200PurposeThe plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsspCas9
TagRFP-T
UseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) eGFP
Plasmid#107505PurposeExpresses eGFP, puromycin resistantDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry
Plasmid#122957PurposeLentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expressionDepositorInsertNOTCH2NLB (NOTCH2NLB Human)
UseLentiviralTags6xmyc tagExpressionMutationPromoterCAG promoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE
Plasmid#20943Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceJuly 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) SDC3
Plasmid#107501PurposeExpresses SDC3, puromycin resistantDepositorInsertSDC3 (codon optimized) (SDC3 Human)
UseLentiviralTagsExpressionMammalianMutationCodon optimized sequencePromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-GABARAPL1 G116
Plasmid#123243PurposeExpression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116. For lentivirus production and stable transduction in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acid 117. Stop codon after G116PromoterPGKAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IKZF1-V1-FLAG-IRES-GFP
Plasmid#107387PurposeLentiviral expression of IKZF1-V1-FLAGDepositorInsertIKZF1-V1 (IKZF1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-gate-3xHA-pGK-PUR
Plasmid#107393PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xHAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-IRF4-FLAG-IRES-GFP
Plasmid#107389PurposeLentiviral expression of IRF4-FLAGDepositorInsertIRF4 (IRF4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-Myc-gate-pGK-HYG
Plasmid#107395PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsMycExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) EPB41L1
Plasmid#107508PurposeExpressing EPB41L1, puromycin resistantDepositorInsertEPB41L1 (EPB41L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti HRE mutant-Luc pGK Hygro
Plasmid#118707Purposereporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferaseDepositorInsertHREmutant-Luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV Puro DEST (w118-1) PHLDB1
Plasmid#107503PurposeExpresses PHLDB1, puromycin resistantDepositorInsertPHLDB1 (PHLDB1 Human)
UseLentiviralTagsExpressionMammalianMutationNucleotide changes 1236 T>C, 1365 C>T, 1371…PromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE
Plasmid#20944Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationH134RPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP
Plasmid#122954PurposeLentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expressionDepositorInsertNOTCH2NLB (NOTCH2NLB Human)
UseLentiviralTags6xhis tagExpressionMutationPromoterCAG promoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-FLAG-CRBN-pGK-HYG
Plasmid#107374PurposeLentiviral expression of FLAG-CRBNDepositorInsertcereblon (CRBN Human)
UseTagsFLAGExpressionMammalianMutationPromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only