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Plasmid#153534PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
retro-gfp-puro vector
Plasmid#58249PurposeRetroviral expression vector encoding bicistronic EGFP and Puromycin cassetteDepositorInsertEGFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD44-isoform1-CD4d3+4-bio
Plasmid#73098PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform1 with rat CD4d3+4-bioDepositorInsertCD44 antigen isoform 1 (CD44 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLS3-5xHRE-p53(K120R/K164R)
Plasmid#72556PurposeHypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.DepositorInsertp53 (TP53 Human)
UseTags5XHREExpressionMammalianMutationK120R/K164RPromoterE1B minimal promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1 double gRNAs
Plasmid#162791PurposeMultiplex Guide RNAs to generate Nr4a1 knockout by CRISPRDepositorInsertMultiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-Farnesyl-5
Plasmid#56159PurposeLocalization: Plasma Membrane, Excitation: 604, Emission: 659DepositorInsertFarnesyl (HRAS Human)
UseTagsmCardinalExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pINSPECT_5'-xrRNA_NLuc_3'-XAP1
Plasmid#197264PurposeCloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-E138A
Plasmid#69921PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationE138APromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CD59-CD4d3+4-bio
Plasmid#73117PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD59 with rat CD4d3+4-bioDepositorUseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP
Plasmid#135176PurposeAAV expression of TRE(TRE3G)-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.DepositorInsertshChR2
TRE3G
UseAAV and Cre/LoxTagsEYFP (C terminal on insert)ExpressionMammalianMutationE123T and T159CPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CD44-isoform12-CD4d3+4-bio
Plasmid#73130PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform12 with rat CD4d3+4-bioDepositorInsertCD44 antigen isoform 12 (CD44 Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-G117S
Plasmid#69920PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationG117SPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-AA
Plasmid#160942PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationChange Cys 287 Glu 288 into Ala AlaPromoterMSCV-LTRAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARD14-D176H
Plasmid#69919PurposeExpresses mutant FLAG-CARD14 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationD176HPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-shBACH1_BFP
Plasmid#199217PurposeExpression plasmid with shRNAmiR targeting BACH1, with a BFP reporter on the backbone.DepositorInsertshBACH1 (BACH1 Synthetic)
UseRNAiTagsExpressionMutationPromoterhuman U6Available sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC_hU6-shRKIP_BFP
Plasmid#199216PurposeExpression plasmid with shRNAmiR targeting RKIP (PEBP1), with a BFP reporter on the backbone.DepositorInsertshBACH1 (BACH1 Synthetic)
UseRNAiTagsExpressionMutationPromoterhuman U6Available sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-TDP-43
Plasmid#162608PurposeAllows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assaysDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-Zf2
Plasmid#160944PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 2 deletion)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationDeletion of Zinc finger 2 (C306 to C330)PromoterMSCV-LTRAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-Zf1
Plasmid#160943PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (zinc finger 1 deletion)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationDeletion of Zinc finger 1 (C270 to C290)PromoterMSCV-LTRAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX303-GFP-IQSEC1 v2N/v4C-WT
Plasmid#162022PurposeExpression of IQSEC1 chimeraDepositorInsertIQSEC1 (IQSEC1 Human)
UseLentiviralTagsExpressionMutationv2N/v4CPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only