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Plasmid#126486PurposeEncodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertLmoCas6-p300
UseCRISPRTagsFlag and Flag, NLSExpressionMammalianMutationPromoterCMV and U6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CasE-p300-Flag
Plasmid#106275PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoterDepositorInsertEcoCasE-p300
UseCRISPRTagsE tag, NLS and FlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
eiABE
Plasmid#227144PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoterDepositorInsertbpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
UseTagsExpressionMutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TadA-8e-eIscBn-C-HMG-D
Plasmid#227145PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoterDepositorInsertbpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
UseTagsExpressionMutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
eIscB-D
Plasmid#227017PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoterDepositorInsertSV40 NLS-HMG-D-linker-OgeuIscB-v3-SV40-nucleoplasmin NLS
UseTagsExpressionMutationD96R/E84R/V159RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TadA-8e-eIscBn
Plasmid#227018PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoterDepositorInsertbpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
UseTagsExpressionMutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
hA3A*-IscBn
Plasmid#227146PurposeVector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoterDepositorInsertbpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
UseTagsExpressionMutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
eiCBE
Plasmid#227148PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoterDepositorInsertbpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
UseTagsExpressionMutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TadA-8e-eIscBn-N-HMG-D
Plasmid#227143PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoterDepositorInsertbpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
UseTagsExpressionMutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_COL4A3_self-cleavingCas9
Plasmid#130281PurposeExpresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivationDepositorInsertspCas9
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_COL4A5_self-cleavingCas9
Plasmid#130282PurposeExpresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivationDepositorInsertspCas9
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-puro
Plasmid#45567DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsIRES-EGFP-PuroExpressionMammalianMutationPromoterPhcmv (strong human cytomegalovirus immediate ear…Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMD2.G-K47Q/R354Q
Plasmid#207323PurposeVSV-G mutant with abolished receptor binding, but maintained fusogenic capacityDepositorInsertVSV-G (VSIVgp4 Vesicular Stomatitis Virus)
UseTagsExpressionMammalianMutationK47Q/R354QPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-EGFP:WTIP
Plasmid#108231PurposeLentiviral vector for Tet-inducible WTIP expressionDepositorInsertWTIP (WTIP Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterTet/ON Minimal CMV promoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRPV1exCellHalo
Plasmid#220307PurposeMammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helicesDepositorInsertrTRPV1exCellHalo (Trpv1 Rat)
UseTagscircularly permutated Halotag (Deo et al., Nat Ch…ExpressionMammalianMutationlinker included to insert cpHaloTag (PromoterCMV promoterAvailable sinceJuly 30, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBMNb-cALFA
Plasmid#159991PurposeFor the protein expression with ALFA tag at C-terminus in mammalian cellsDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationCMV promoter, WPREPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-tTA
Plasmid#162598Purposehuman CD81-TEV cleavage site-tTADepositorInserthuman CD81 (CD81 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetOn-mCherry-eGFP-RAMP4
Plasmid#109014PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)DepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NP-B/BRISBANE/60/2008
Plasmid#187921PurposeExpresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cellsDepositorInsertInfluenza B Nucleoprotein
UseTagsUNTAGGEDExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZW1-FCS-circRNA
Plasmid#73449PurposeExpression of circular RNADepositorInsertPOLR2A (POLR2A Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMV promoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only