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Plasmid#156448PurposeFor transient expression of human CTCF tagged with eGFPDepositorInserthuman CTCF (CTCF Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry
Plasmid#75470PurposeFlp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertReversed ChR2(H134R)-mCherry
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine
Plasmid#228902PurposeFor AAV packaging and Cre dependent expression of a peripherally restricted DREADDDepositorInserthydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
UseAAV and Synthetic BiologyTagsHA and P2A mCitrineExpressionMammalianMutationR108KPromoterChicken Beta ActinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs
Plasmid#220493PurposeTo generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.DepositorInsertsgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP
Plasmid#129111PurposeHomologous recombination vector for excision reporter 1 in the CLYBL locus.DepositorInsertLSL, eGFP, NLS-RFP
UseCre/Lox and TALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA
Plasmid#156445PurposeFor transient expression of mouse RAD21 (SCC1) tagged with mKate2DepositorInsertmouse RAD21 (Rad21 Mouse)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-gate-FLAG-IRES-GFP
Plasmid#107398PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)
Plasmid#140001PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9-NG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChR2(H134R)-eYFP
Plasmid#127090PurposeCre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activationDepositorHas ServiceAAV PHP.eBInsertChannelrhodopsin-2
UseAAVTagseYFP (C terminal on insert)ExpressionMammalianMutationHumanized ChR2 gene with histidine 134 changed to…PromoterCAGAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027
Plasmid#134611PurposeMammalian expression of mouse KIF5A 378-1027DepositorInsertKIF5A (Kif5a Mouse)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955
Plasmid#134613PurposeMammalian expression of rat KIF5C 378-955DepositorInsertKIF5C (Kif5c Rat)
UseTagsHaloExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)
Plasmid#139998PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCAGAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE
Plasmid#162382PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor
Plasmid#92142PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE
Plasmid#162380PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8s
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationS26M F286Y Q315HPromoterCAGAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSH-CAG-AtAFB2.F74A-IRES-puro
Plasmid#216245PurposeExpress AtAFB2.F74A mutant without tag for AID2DepositorInsertAtAFB2.F74A
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsExpressionMammalianMutationF74A mutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA
Plasmid#183776PurposeAAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.DepositorInsertCre
UseAAVTagsExpressionMutationNAPromoterCAGAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE
Plasmid#162381PurposeAAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8m
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationA25G F286YPromoterCAGAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203229PurposeCre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertCre-dependent, membrane-localized Optopatch
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cas9-T2A-EGFP-ires-puro
Plasmid#78311PurposeExpresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.DepositorInsertWT SpCas9-T2A-EGFP-ires-puromycin resistance
UseCRISPRTagsT2A-EGFP-ires-puroExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only