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Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
UseTags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK621
Plasmid#196405PurposeStrong expression of firefly luciferase codon-optimized for S. rosettaDepositorInsertSro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine
Plasmid#197681Purpose"Insert(high)-pEF1s(orig)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine
Plasmid#197683Purpose"Insert(low)-pEF1s(orig)" reporter plasmidDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
vYM142_phiC31-nTRE-pEF1s(high)(CC793)-H2B-citrine
Plasmid#197685Purpose"pEF1s(high)" reporter plasmid, with CpG793 mutated to CCDepositorInsertpEF1s-H2B-Citrine
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4E-I96AI112A-siRNAres_Z
Plasmid#148163PurposeInsect Expression of DmeIF4E-I96AI112ADepositorInsertDmeIF4E-I96AI112A
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4E-W106A-siRNAres_Z
Plasmid#148164PurposeInsect Expression of DmeIF4F-W106ADepositorInsertDmeIF4F-W106A
UseTagsExpressionInsectMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-DmeIF4E_69-248_Z
Plasmid#148172PurposeBacterial Expression of DmeIF4E_69-248DepositorInsertDmeIF4E_69-248
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpH-DmeIF4E_69-248-I96AI112A_Z
Plasmid#148173PurposeBacterial Expression of DmeIF4E_69-248-I96AI112ADepositorInsertDmeIF4E_69-248-I96AI112A
UseTagsExpressionBacterialMutation3 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-FWK41AAA_T
Plasmid#147603PurposeInsect Expression of Dm4E-T-FKW41AAADepositorInsertDm4E-T-FKW41AAA (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-Y10AL15A_T
Plasmid#147605PurposeInsect Expression of Dm4E-T-Y10AL15ADepositorInsertDm4E-T-Y10AL15A (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T-4xmut_T
Plasmid#147606PurposeInsect Expression of Dm4E-T-4xmutDepositorInsertDm4E-T-4xmut (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T-5xmut_T
Plasmid#147607PurposeInsect Expression of Dm4E-T-5xmutDepositorInsertDm4E-T-5xmut (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T-FWK41AAA_T
Plasmid#147611PurposeInsect Expression of Dm4E-T-FWK41AAADepositorInsertDm4E-T-FWK41AAA (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T-L36AL39A_T
Plasmid#147612PurposeInsect Expression of Dm4E-T-L36AL39ADepositorInsertDm4E-T-L36AL39A (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-NRE90AAA_T
Plasmid#147577PurposeInsect Expression of Dm4E-T-NRE90AAADepositorInsertDm4E-T-NRE90AAA (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4E-T-NRE90AAA_T
Plasmid#147582PurposeInsect Expression of Dm4E-T-NRE90AAADepositorInsertDm4E-T-NRE90AAA (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-4xmut_T
Plasmid#147593PurposeInsect Expression of Dm4E-T-4xmutDepositorInsertDm4E-T-4xmut (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-5xmut_T
Plasmid#147594PurposeInsect Expression of Dm4E-T-5xmutDepositorInsertDm4E-T-5xmut (4E-T Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2727
Plasmid#193330PurposeSite specific insertion using CRISPR/Cas9 editing of C. elegans ChrIDepositorInsertHygromycin resistance
UseTagsExpressionWormMutationPromoterPrps-0Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only