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Plasmid#127845Purposeclone A3A CBEs by SapI Golden GateDepositorInsertA3A
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJB001 CMV 3xFLAG-NLS-FKBP-ZF2
Plasmid#161545PurposeConstitutive expression of 3xFLAG-NLS-FKBP-ZF2 under the CMV promoterDepositorInsert3xFLAG-NLS-FKBP-ZF2
UseSynthetic BiologyTags3x-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtPT4Pro
Plasmid#159414PurposeMedicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtPT4 Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtBCP1Pro
Plasmid#159415PurposeMedicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtBCP1 Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2
Plasmid#182348PurposemScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-GPXC71S
Plasmid#191703PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701DepositorInsertchl-redox(ro)GFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I35 p35S-XVE-pEstradiol GG0-GG1
Plasmid#195904PurposeLevel 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)DepositorInsertp35S-XVE-LexA-p35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2259 - Intron GG2 - 900 bp
Plasmid#159881PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - 900 bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2
Plasmid#182345PurposeGFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT100.110
Plasmid#122602Purposeclone CDA1 phage by SapI Golden GateDepositorInsertCDA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1359 - Intron GG2 - smu-1
Plasmid#159805PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT362
Plasmid#127844Purposeclone BE4max-NG CBEs by SapI Golden GateDepositorInsertBE4max-NG without deaminase
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2215 - Intron GG3 - 900 bp
Plasmid#159882PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - 900 bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
EC71022
Plasmid#159608PurposeLevel 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxPDepositorInsertloxP-mCherry-HDEL-t35S-loxP
UseCre/Lox and Synthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pShoHELIX-sgRNA_entry (BO3)
Plasmid#181784PurposeExpresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL11023
Plasmid#117542PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S_OMEGA(pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT100.136c
Plasmid#122603Purposeclone FERNY phage by SapI Golden GateDepositorInsertFERNY
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1360 - Intron GG3 - smu-1
Plasmid#159806PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG3 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1358 - Intron GG1 - smu-1
Plasmid#159804PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - smu-1
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2214 - Intron GG1 - 900 bp
Plasmid#159880PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - 900 bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only