We narrowed to 3,754 results for: golden gate

1. pCHW00019

   Plasmid

   #222448

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   GAL4AD-NLS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCHW100003

   Plasmid

   #222442

   Purpose

   Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   mCherry2-HyP5SM
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCHW111114

   Plasmid

   #222459

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-CMV1 (Cucumber mosaic virus)

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCHW111118

   Plasmid

   #222461

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-CMV1 (Cucumber Mosaic Virus)

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCHW111113

   Plasmid

   #222458

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-CMV1 (Cucumber mosaic virus)

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCHW91003

   Plasmid

   #222449

   Purpose

   Level 1, Position 3 containing 35S::RUBY::OCS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   35S::RUBY::OCS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCHW91070

   Plasmid

   #222463

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-CMV1::mCherry2-HyP5SM-LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-CMV1 (Cucumber Mosaic Virus)

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYPQ145

   Plasmid

   #69297

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 5 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJB158

   Plasmid

   #202601

   Purpose

   RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

   Depositor

   Athanasios Typas

   Article

   Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH220

    Plasmid

    #122836

    Purpose

    dCas9 with GreenGate B-C flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    

    Use

    Golden gate compatible cloning vector

    Mutation

    Contains D10A and H840A mutations

    Available Since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPOM033_P7_3"His5

    Plasmid

    #216458

    Purpose

    3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    3''his5
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPOM026_P2_pNMT1

    Plasmid

    #216451

    Purpose

    Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter nmt1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPOM019_P2_pRPL2102

    Plasmid

    #216444

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter rpl2102
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPOM015_P2_pADH1

    Plasmid

    #216440

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter adh1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPOM013_P2_pENO101

    Plasmid

    #216438

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter eno101
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ148

    Plasmid

    #69300

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCHW00016

    Plasmid

    #222447

    Purpose

    Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    p65AD-NLS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCBLsgRNA1

    Plasmid

    #199727

    Purpose

    Golden Gate entry vector; 1st gRNA under OsU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU3-sgRNA1 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCBLsgRNA2

    Plasmid

    #199728

    Purpose

    Golden Gate entry vector; 2nd gRNA under OsU3 promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    OsU3-sgRNA2 scaffold
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGGF012

    Plasmid

    #48849

    Purpose

    Provides a sulfadiazine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pMAS::SulfadiazineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition sites in SulfR removed …

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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