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Showing: 1901 - 1920 of 31395 results

  3. pDG458

    Plasmid
    #100900
    Purpose
    SpCas9 with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Availability
    Academic Institutions and Nonprofits only
    100900-plasmid-map-sequence-id-196982

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  4. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  17. pCAG-eCas9-GFP-U6-gRNA

    Plasmid
    #79145
    Purpose
    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cells
    Depositor
    Jizhong Zou
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    79145-plasmid-map-sequence-id-182555

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  18. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  19. pTP63-donor

    Plasmid
    #132477
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TP63 (TP63 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    132477-plasmid-map-sequence-id-273771

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  20. pID3-donor

    Plasmid
    #176342
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ID3 (ID3 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    176342-plasmid-map-sequence-id-347890

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Showing: 1901 - 1920 of 31395 results