Addgene

We narrowed to 51,996 results for: cloning

Showing: 1901 - 1920 of 51996 results

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Rabbit SERCA2b (pBSII-SK-)

Plasmid

#75186

Purpose

Cloning vector for ATP2A2

Depositor

Jonathan Lytton, David MacLennan

Article

Lytton et al J Biol Chem. 1989 Apr 25;264(12):7059-65

Insert

ATP2A2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pYEXC3H

Plasmid

#49026

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD008

Plasmid

#48838

Purpose

Provides an ER retrieval signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

ER retrieval signal

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB002

Plasmid

#48820

Purpose

Provides the omega element as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

Omega element

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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MXS_P2A

Plasmid

#62433

Purpose

MXS_chaining vector with P2A

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Self-cleaving peptide

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_H2B

Plasmid

#62432

Purpose

MXS_chaining vector with H2B

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Histone 2B with leading Kozak sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGGEV_2_+nls-eGFP-HA-_+1_BK+

Plasmid

#49312

Purpose

Golden Gate entry vector: Position 2, HA tagged nls-EGFP with start and stop codon

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

nls-EGFP-HA

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pcDXC3GMS

Plasmid

#49031

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGGH000

Plasmid

#48862

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag + terminator modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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R777-E116 Hs.KRAS-nostop

Plasmid

#70400

Purpose

Gateway ORF clone of human KRAS [NM_004985.4] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBXNAC3H

Plasmid

#47074

Purpose

FX cloning E.coli expression vector with araBAD promoter N-terminal Avi-tag, C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), Avi-tag, and…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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MXS_MCS3

Plasmid

#62397

Purpose

MXS_chaining vector with MCS3

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

MluI-XhoI-HindIII-PstI-SalI MCS

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pPOM012_P5_ConR11

Plasmid

#216437

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM011_P5_ConR10

Plasmid

#216436

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM010_P5_ConR9

Plasmid

#216435

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R9

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM009_P5_ConR8

Plasmid

#216434

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R8

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM008_P5_ConR7

Plasmid

#216433

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R7

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM007_P5_ConR6

Plasmid

#216432

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 5 following the YeastToolkit MoClo grammar. Right connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector R6

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM006_P1_ConL11

Plasmid

#216431

Purpose

Allows multigene assembly with MoClo golden gate cloning. Part type 1 following the YeastToolkit MoClo grammar. Left connector.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Connector L11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge