Addgene

We narrowed to 13,309 results for: cas9

Showing: 1901 - 1920 of 13309 results

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AID-C12*-n'Cas9-BE4max

Plasmid

#216730

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-n'Cas9-BE4max (AICDA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-MYC-Guide-hSpCas9

Plasmid

#228574

Purpose

Expression of human MYC gRNA; CRISPR mediated gene insertion

Depositor

Marcin Imielinski

Insert

MYC gRNA (MYC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-sgAMPKa2-Cas9-GFP

Plasmid

#208050

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa2

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-sgAMPKa1-Cas9-GFP

Plasmid

#208049

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2699-CMV-Nme2Cas9-E932R

Plasmid

#228720

Purpose

Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-E932R nuclease

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9 E932R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2696-CMV-Nme2SmuCas9-D56R

Plasmid

#228717

Purpose

Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-D56R nuclease

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D56R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2698-CMV-Nme2Cas9-D56R

Plasmid

#228719

Purpose

Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-D56R nuclease

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9 D56R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH-ABE8.17-m+V106W

Plasmid

#198551

Purpose

Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH-ABE8.17-m+V106W

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-E690_guide-CBh-hSpCas9

Plasmid

#188545

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-W308_guide-CBh-hSpCas9

Plasmid

#188546

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-L101_guide-CBh-hSpCas9

Plasmid

#188547

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Expression

Mutation

Promoter

U6

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SauriCas9-KKH-BlastR

Plasmid

#220972

Purpose

Expresses human codon-optimized SauriCas9-KKH

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9-KKH

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pME-dCas9-BFP-KRAB

Plasmid

#221502

Purpose

CRISPRi machinery fish codon optimized

Depositor

Alessandro Ori

Insert

dCas9-tagBFP-KRAB

Use

Fish expression

Tags

Expression

Mutation

D10A and H839A point mutations

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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mPLD3-sgRNA-Cas9-mcherry

Plasmid

#199700

Purpose

encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

Depositor

David Nemazee

Insert

hSpCas9

Use

CRISPR

Tags

P2A-RFP

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-SpCas9D10A nickase

Plasmid

#216736

Purpose

Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

Depositor

Vincent Dion

Article

Alvaro et al bioRxiv

Insert

Cas9-D10A nickase

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

EF-1-alpha core

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AID-C12*-dCas9-BE4max

Plasmid

#216731

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-dCas9-BE4max (AICDA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9HF1-P2A-puro

Plasmid

#214357

Purpose

Expresses SpCas9HF1 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9HF1 and puromycin resistance genes

Use

Tags

Expression

Mammalian

Mutation

N497A/R661A/Q695A/Q926A

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugCas9-P2A-puro

Plasmid

#214353

Purpose

Expresses SlugCas9 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugCas9 and puromycin resistance genes

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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