Addgene

We narrowed to 34,748 results for: KAN

Showing: 1901 - 1920 of 34748 results

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ptCMV-153/47-VR-NN

Plasmid

#50706

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tipin-E185A

Plasmid

#23114

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Glutamate 185 to Alanine

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-Flag-Tipin-E190A

Plasmid

#23115

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed Glutamate 190 to Alanine

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pET21b-Tipin-His-L195A

Plasmid

#23123

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

His

Expression

Bacterial

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pBK1340-LV-DUAL-dGFP-NanoLuc-Puro

Plasmid

#223160

Purpose

Expression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-life

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

dGFP-P2A-NanoLuc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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hSUMO1 GFP

Plasmid

#118360

Purpose

This plasmid expresses the full length human SUMO1.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hSUMO1 (SUMO1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSN837

Plasmid

#184825

Purpose

Expresses human KIF5A in mammalian cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (KIF5A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

Plasmid

#121184

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

CMV-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Tags

Expression

Mutation

OsTIR1 codon optimized for human cells

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-ORAI1-EC-HA

Plasmid

#174335

Purpose

encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Tags

GFP in the N-terminus and HA tag in the extracell…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSN838

Plasmid

#184826

Purpose

Expresses human KIF5A(∆exon27) in mammalian cells

Depositor

Kyoko Chiba

Article

Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

Insert

KIF5A (∆exon27) (KIF5A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

∆exon27 form

Promoter

CMV

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-Actinin-19

Plasmid

#57346

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pN1-mCherry-NKD2

Plasmid

#176641

Purpose

Expresses NKD2-mCherry fusion protein in mammalian cells

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

mCherry tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-Alpha-Actinin-C-14

Plasmid

#57347

Purpose

Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Alpha-Actinin (ACTN1 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac-His-Flag-Tipin-L195A

Plasmid

#23120

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

Flag- and His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Tipin-L195A

Plasmid

#23121

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Tipin (TIPIN Human)

Use

Tags

His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pBK1289-AAV-dCjCas9

Plasmid

#223138

Purpose

AAV backbone for dCjCas9 (endonuclease dead Cas9 from Campylobacter jejuni) with Cj-gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1290-AAV-dSaCas9

Plasmid

#223139

Purpose

AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Combined γB2FL-FRET probe

Plasmid

#209094

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 full length (FL)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Combined γB2ΔICD-FRET probe

Plasmid

#209093

Purpose

mTurquoise2- and mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Hoshino et al Proc Natl Acad Sci U S A. 2023 Sep 19;12

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Use

Tags

PA tag

Expression

Mammalian

Mutation

Promoter

Chicken β-actin

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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