-
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAT77
Plasmid#204295PurposepJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_Tmcs
Plasmid#25751PurposeEntry vector with TRE promoter followed by multiple cloning site.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c_ddCpf1_final
Plasmid#212040PurposeBacterial expression of the CRISPRi system for genome editing in E. coliDepositorInsertddCpf1
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6d-IVT
Plasmid#215836PurposeTemplate for in vitro transcription of CBE6d (with SpCas9)DepositorInsertCBE6d-SpCas9-2xUGI
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT21
Plasmid#204309PurposeFor generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthpartsDepositorInsertie1-GFPn
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT25
Plasmid#204310PurposeFor generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.DepositorInsertie1-GFPc
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mSA-phi29
Plasmid#210815Purposein vitro transcription of mRNADepositorInsertmSA-Phi29
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHO-UAS-mScarlet-I
Plasmid#183147PurposeYeat two-hybrid fluorecent reporter for genomic integrationDepositorInsertmScarlet-I
UseTagsExpressionMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z31
Plasmid#120074PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5DepositorInsertV5
UsePcr templateTagsV5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCarmine in pRSETB
Plasmid#109484Purposebacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)DepositorInsertmCarmine
UseTagsN-terminal histidine tag in vectorExpressionBacterialMutationPromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2SK
Plasmid#59383PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.DepositorInsertTT-ISceI-kan
UseTemplateTagsExpressionMutationPromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAT43
Plasmid#204299PurposeFor introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24-1A(sfGFP)
Plasmid#126971PurposeLevel 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEX128·gRNA
Plasmid#187600PurposeTemplate for amplification of gRNA with Cas6 recognition siteDepositorInsertgRNA template
UseTagsExpressionMutationPromoterno promoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28c-Ku
Plasmid#161936PurposeExpresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purificationDepositorInsertku (mku Mycobacterium tuberculosis)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT27
Plasmid#204308PurposeFor generating targeting inversions. C-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPc
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT18
Plasmid#204307PurposeFor generating targeting inversions. N-terminal split GFP, expressed in flight muscles.DepositorInsertMHC-GFPn
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
IVT-MCP-MMLV-RT
Plasmid#207457Purposein vitro transcription of mRNADepositorInsertMCP-RT
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only