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Plasmid#80874Purposemammalian expression of ALK3 Q233DDepositorInsertALK3 (BMPR1A Human)
UseTagsHAExpressionMammalianMutationQ233D (constitutively active)PromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-I-PhoCl-HCVp
Plasmid#87697PurposeExpress I-PhoCl-HCVp with N-term EBFP2 in mammalian cellsDepositorInsertI-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
UseTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
UseTagsV5 Epitope TagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2(WT)-8his
Plasmid#149268PurposeMammalian expression plasmid for his-tagged sACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-D…PromoterCMVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 proHB-EGF CS
Plasmid#11601DepositorInsertHB-EGF (HBEGF Human)
UseTagsHis and mycExpressionMammalianMutationConstitutively secreted HB-EGF: C-terminal amino …PromoterAvailable sinceApril 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-TORCAR
Plasmid#64929PurposeLysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.DepositorInsertLAMP1-TORCAR (LAMP1 Synthetic, Human)
UseTagsCerulean, Full-length LAMP1, and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 myc-RNF114
Plasmid#58295Purposemammalian expression of myc tagged RNF114DepositorInsertRNF114 (RNF114 Human)
UseTagshis and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro
Plasmid#208646PurposepcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.DepositorTypeEmpty backboneUseCRISPRTagsClawed frog nucleoplasmin NLS, Flag, Puromycin re…ExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-K179M
Plasmid#73409PurposeExpresses kinase-inactive mutant of Akt with HA tagDepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationchanged K179M to make Kinase inactive mutantPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ALK-V5/HIS
Plasmid#200115PurposeExpression of human ALK receptor tyrosine kinase in mammalian cellsDepositorInserthuman ALK receptor tyrosine kinase (ALK Human)
UseTagsV5/HisExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2
Plasmid#192440PurposeEncodes mCherry-tagged wild-type TSC2 isoform 5.DepositorInsertmCherry-TSC2 (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM4D(Gi)
Plasmid#45548DepositorInsertHA-hM4D
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF7(5’UTR)-FF
Plasmid#85490PurposeFirefly luciferase under the control of IRF7 5'UTRDepositorInsert5'UTR of IRF7 (Irf7 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma1-GFP2
Plasmid#140989PurposeEncodes a G gamma subunit (GNGT1) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma1-GFP2 (GNGT1 Human)
UseTagsGFP2 and GSAG linkerExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Beclin1 1-242
Plasmid#24391DepositorInsertBeclin1 (BECN1 Human)
UseTagsFLAGExpressionMammalianMutationContains amino acids 1-242.PromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDH2-R172K
Plasmid#87927PurposeExpresses IDH2 (R172K mutant) in mammalian cellsDepositorInsertisocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
UseTagsnoneExpressionMammalianMutationR172KPromoterCMVAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-STAT3-S727D
Plasmid#73364PurposeExpression of STAT3-S727DDepositorInsertSignal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
UseTagsFLAG taggedExpressionMammalianMutationE16K S727DPromoterCMVAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA HA FAK Y925F
Plasmid#50509DepositorInsertprotein tyrosine kinase, mutated
UseTagsHAExpressionMammalianMutationY925FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_Lambda-N-HA-Peptide
Plasmid#92005PurposeExpresses Lambda-N-HA peptideDepositorInsertLambda-N-HA peptide
UseTagsLambda-N-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-ORF69-2xCSTREP
Plasmid#136230PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF69DepositorInsertORF69
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only