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Plasmid#204177PurposepRegev_S5 with double mutations that restore secondary structure.DepositorInsertExon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutation49C>A and 59G>U mutationsPromoterTruncated CAGAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO5
Plasmid#193749PurposeExpresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selectionDepositorInserttdTomato
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO3
Plasmid#193748PurposeExpresses E2-Crimson under the control of PAAH synthetic promoter regulated by AHL and aTc, p15A origin of replication, Chloramphenicol selectionDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPAAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pO6
Plasmid#193747PurposeExpresses E2-Crimson under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection,DepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterPIAHAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR
Plasmid#193746PurposeExpresses LuxR under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selectionDepositorInsertLuxR
UseTagsExpressionBacterialMutationPromoterBBa_J23102Available sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-matDYK
Plasmid#115260PurposeFor mammalian expression of a human NODAL splice varaint open reading frame with an internal DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-matNODALvar-HIS
Plasmid#115265PurposeFor bacterial recombinant protein production of the mature peptide of a human NODAL splice variantDepositorInsertNODAL variant mature peptide (NODAL Human)
UseTags6HisExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-MYC-DYK
Plasmid#115245PurposeFor mammalian expression of a human NODAL splice variant open reading frame with a C-terminal MYC-DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR
Plasmid#178905Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-iGFP-DRS2
Plasmid#177104PurposeExpresses iGFP-Drs2 in yeast cellsDepositorInsertDRS2 (DRS2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-Hygro hTERT dEx2-nanoLuc
Plasmid#171056PurposehTERT dEx2-expressing splicing minigeneDepositorInserthTERT dEx2-nanoLuc
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-AUR1-msGFP2
Plasmid#177101PurposeExpresses Aur1-msGFP2 in yeast cellsDepositorInsertAUR1 (AUR1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RBD2-6xmGFP
Plasmid#177109PurposeExpresses Rbd2-6xmGFP in yeast cellsDepositorInsertRBD2 (RBD2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-SEC2-msGFPx3
Plasmid#177110PurposeExpresses Sec2-msGFPx3 in yeast cellsDepositorInsertSEC2 (SEC2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2b
Plasmid#164964PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2b (LOC565691 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJF144_SECGFP_sg2
Plasmid#163865PurposesgRNA targeting GFP in C. elegans. Backbone: pJJR50.DepositorInsertsgRNA targeting GFP
UseCRISPRTagsExpressionWormMutationPromoterR07E5.16 (U6)Available sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnma1b
Plasmid#164952PurposeZebrafish kcnma1b gene CDSDepositorInsertkcnma1b (kcnma1b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb2a
Plasmid#164953PurposeZebrafish kcnmb2a gene CDSDepositorInsertkcnmb2a (kcnmb2 Zebrafish)
UsePcr cloning vectrorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb2b
Plasmid#164954PurposeZebrafish kcnmb2b gene CDSDepositorInsertkcnmb2b (si:ch211-247n2.1 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnmb3
Plasmid#164955PurposeZebrafish kcnmb3 gene CDSDepositorInsertkcnmb3 (si:ch211-38m6.7 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only