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Plasmid#155078PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectivelyDepositorInsert(hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-5xA
Plasmid#92010PurposeExpresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseTagsLambda-N-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-p18Ink4c-IRES-GFP
Plasmid#52220Purposeretroviral bicistronic expression of p18Ink4c and GFPDepositorInsertInk4c (Cdkn2c Mouse)
UseRetroviralTagsIRES-GFPExpressionMammalianMutationPromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+_LN-HA-AGO2-5xE
Plasmid#92011PurposeExpresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]DepositorInsertAGO2 (AGO2 Human)
UseTagsLambda-N-HAExpressionMammalianMutationS824E, S828E, T830E, S831E, S834E (5xE)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR8/GW/TOPO-ELF1
Plasmid#98627PurposeGateway entry TOPO TA cloning vector ELF1DepositorInsertELF1 (Elf1 Mouse)
UseTopo ta cloning vectorTagsExpressionMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4C03
Plasmid#70961PurposeGateway entry cloneDepositorInsertHis2Av (His2Av Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_TK1_HPRT1_Lb_4
Plasmid#155062PurposeLentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)DepositorInsert(hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Alpha2 delta-1 V6
Plasmid#89894PurposeMammalian expression of Alpha2 delta-1 V6DepositorInsertCacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationAmino acids L943V, E944V, A945V, E947V, M948V are…Promoteradenovirus major late promoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
BAM3 X078_pECIA2
Plasmid#115106PurposeBait vector BAM3 X078_pECIA2 should be used with prey vector BAM3 X078_pECIA14.DepositorInsertAT4G20270 (AT4G27020 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
RGFR3 X052_pECIA2
Plasmid#115107PurposeBait vector RGFR3 X052_pECIA2 should be used with prey vector RGFR3 X052_pECIA14.DepositorInsertAT4G26540 (AT4G26540 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT4G22730 K11_pECIA2
Plasmid#115024PurposeBait vector AT4G22730 K11_pECIA2 should be used with prey vector AT4G22730 K11_pECIA14.DepositorInsertAT4G22730 (AT4G22730 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT4G23740 J11_pECIA2
Plasmid#114998PurposeBait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.DepositorInsertAT4G23740 (AT4G23740 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G37050 N3_pECIA2
Plasmid#114952PurposeBait vector AT2G37050 N3_pECIA2 should be used with prey vector AT2G37050 N3_pECIA14.DepositorInsertAT2G37050 (AT2G37050 Mustard Weed)
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
RGFR3 X052_pECIA14
Plasmid#114907PurposePrey vector RGFR3 X052_pECIA14 should be used with bait vector RGFR3 X052_pECIA2.DepositorInsertAT4G26540 (AT4G26540 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT4G22730 K11_pECIA14
Plasmid#114824PurposePrey vector AT4G22730 K11_pECIA14 should be used with bait vector AT4G22730 K11_pECIA2.DepositorInsertAT4G22730 (AT4G22730 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT4G23740 J11_pECIA14
Plasmid#114798PurposePrey vector AT4G23740 J11_pECIA14 should be used with bait vector AT4G23740 J11_pECIA2.DepositorInsertAT4G23740 (AT4G23740 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AT2G37050 N3_pECIA14
Plasmid#114752PurposePrey vector AT2G37050 N3_pECIA14 should be used with bait vector AT2G37050 N3_pECIA2.DepositorInsertAT2G37050 (AT2G37050 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-SNCAe3-WT
Plasmid#85847PurposeDonor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomatoDepositorInsertSNCA exon 3 homology arms (SNCA Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHMP3-mut-NS3-Green
Plasmid#223530PurposeMammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.DepositorInsertCHMP3 (CHMP3 Human)
UseTagsNS3 Hepatitis C protease and mNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
ADORA2A-DuET
Plasmid#213174PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertADORA2A (ADORA2A Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only