Addgene

We narrowed to 18,998 results for: tes

Showing: 1861 - 1880 of 18998 results

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pRK5 HA-GST-Presc-mAkt1 (S473T)

Plasmid

#48806

Purpose

expresses mutant Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Use

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

S473T

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pM-ErbB4-delta-kinase-PY3 mutant

Plasmid

#17802

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pDONR221-In18runon-PTPN22

Plasmid

#195377

Purpose

A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Intron 18-runon PTPN22

Promoter

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-In18runon-PTPN22

Plasmid

#195379

Purpose

Mammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Intron 18-runon PTPN22

Promoter

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Ex1-17-PTPN22

Plasmid

#195375

Purpose

Mammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

Insert

PTPN22 (PTPN22 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

PTPN22 mutant containing exon 1 to 17

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pPhiC31-actIIORF4

Plasmid

#190785

Purpose

The PhiC31-phage based integration vector containing the actII-ORF4 gene under the control of strong kasOp* promoter

Depositor

Jan Kormanec

Article

Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

Inserts

T5 terminator
actII-4
kasOp*

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-ALDH1A3gRNA3

Plasmid

#189748

Purpose

The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.EPC1

Plasmid

#184653

Purpose

Retroviral expression of mouse Epc1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc1 (Epc1 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-MYC.Mm.EPC2

Plasmid

#184654

Purpose

Retroviral expression of mouse Epc2

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Epc2 (Epc2 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(V303R)-IRES-puro

Plasmid

#128659

Purpose

mammalian expression of Flag-Polß(V303R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

V303R

Promoter

CMV

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K72A)-IRES-puro

Plasmid

#128661

Purpose

mammalian expression of Flag-Polß(K72A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K72A

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-copGFP-Polß(WT)-puro

Plasmid

#128665

Purpose

mammalian expression of copGFP-Polß(WT) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K244A)-IRES-puro

Plasmid

#128682

Purpose

mammalian expression of Flag-Polß(K244A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K244A

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R)-IRES-puro

Plasmid

#128654

Purpose

mammalian expression of Flag-Polß(L301R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(K206A)-IRES-puro

Plasmid

#128678

Purpose

mammalian expression of Flag-Polß(K206A) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K206A

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(V306R)-IRES-puro

Plasmid

#128655

Purpose

mammalian expression of Flag-Polß(V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

V306R

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pDRf1-4CL5-AtSDT (JBEI-11535)

Plasmid

#87934

Purpose

Co-expression in S.cerevisiae of 4CL5 and AtSDT

Depositor

Dominique Loque

Article

Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

Inserts

SDT (SDT Mustard Weed)
4CL5 (4CL5 Mustard Weed)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pM-ErbB4-delta-kinase-PY1 mutant

Plasmid

#17801

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Use

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
HIP-flash (HOP-flash mutant)

Plasmid

#83466

Purpose

Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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