Addgene

We narrowed to 14,448 results for: invs

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MSCV-dGFP-JARID1C A388P

Plasmid

#74778

Purpose

retroviral expression of JARID1C A388P

Depositor

Giovanni Tonon

Article

Rondinelli et al J Clin Invest. 2015 Dec;125(12):4625-37.

Insert

JARID1C (KDM5C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

A388P

Promoter

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-Smad2

Plasmid

#89827

Purpose

Knockdown human Smad2 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD2 shRNA (SMAD2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-Fibrillarin-7

Plasmid

#56160

Purpose

Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Fibrillarin (FBL Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGLS3-5xHRE-p53(K164R)

Plasmid

#72555

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K164R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Use

Tags

5XHRE

Expression

Mammalian

Mutation

K164R

Promoter

E1B minimal promoter

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGLS3-5xHRE-p53(K120R)

Plasmid

#72554

Purpose

Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

p53 (TP53 Human)

Use

Tags

5XHRE

Expression

Mammalian

Mutation

K120R

Promoter

E1B minimal promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry IL36RN S113L

Plasmid

#58321

Purpose

entry vector for human IL36RN S113L mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Expression

Mutation

S113L: c.338C>T, p.Ser113Leu

Promoter

CMV

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pLEX301-RFP-GGA3 NGAT

Plasmid

#162031

Purpose

Expresses NGAT sequence of GGA3

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

GGA3 NGAT (GGA3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-135

Plasmid

#31067

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-135 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV6-Entry IL36RN R102W

Plasmid

#58323

Purpose

entry vector for human IL36RN R102W mutant

Depositor

Francesca Capon

Article

Hassi et al J Invest Dermatol. 2023 Dec;143(12):2468

Insert

IL36RN (IL36RN Human)

Use

Entry vector

Tags

myc-DDK

Expression

Mutation

R102W: c.304C>T, p.Arg102Trp

Promoter

CMV

Available since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pECE-M2-CDC27 S379A

Plasmid

#31662

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog S379A (CDC27 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

changed Serine 379 to Alanine

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
HNF4A_P2-226

Plasmid

#31064

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-226 to -1 of P2 HNF4A promoter

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-CDC27 S379A

Plasmid

#31674

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog S379A (CDC27 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

changed Serine 379 to Alanine

Promoter

Available since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pHR-CLIP-CD28(CD45TM)

Plasmid

#223629

Purpose

Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Expression

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(CD45TM)

Plasmid

#223624

Purpose

Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Expression

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD86(F56A)

Plasmid

#223609

Purpose

Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

F56A

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD80(Y65A)

Plasmid

#223608

Purpose

Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Y65A

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(SGGG)

Plasmid

#223607

Purpose

Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Expression

Mutation

MYPPPY mutated to SGGG

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-(-1899)

Plasmid

#202514

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -1899 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pUt-mNF-GFP

Plasmid

#199220

Purpose

LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

hTetR::P2A::EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV D2ir promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pUt-mPF-GFP

Plasmid

#199222

Purpose

LPUtopia-7 matching RMCE donor plasmid with GFP Positive-Feedback circuit (mPF-GFP). Use BlastR for positive selection and HSV-TK for negative selection.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Insert

rtTA-Advanced::P2A::EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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