Addgene

We narrowed to 33,274 results for: eng

Showing: 1861 - 1880 of 33274 results

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pICH71311

Plasmid

#50282

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter and 5'UTR, RbcS3A (S. lycopersicum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00001

Plasmid

#50346

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50012

Plasmid

#50311

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal V5 tag (Simian Virus 5)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50013

Plasmid

#50312

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal T7 tag (bacteriophage T7 gene 10)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH71301

Plasmid

#50281

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter and 5'UTR, RbcS2 (S. lycopersicum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH37431

Plasmid

#50306

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

signal peptide, RAmy3A (O. sativa)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45089

Plasmid

#50254

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH42760

Plasmid

#50257

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, spm (Z. mays)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE003

Plasmid

#48854

Purpose

Provides the At1g04880 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g04880 terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC014

Plasmid

#48827

Purpose

Provides GFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

A206K

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2h-IRES-mCherry-CAAX

Plasmid

#208691

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2h

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_N

Plasmid

#209553

Purpose

pCMV-bpSV40 NLS-TadA8e(2-76)-2xFRB-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

TadA-8e(2-76)-2xFRB

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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sABE v3.22_C

Plasmid

#209554

Purpose

pCMV-SV40_NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A)-SV40_NLS-BGHpA

Depositor

Xue Gao

Article

Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

Insert

2xFKBP-TadA-8e(77-167)-SpCas9(D10A)

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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TRAIP-SFB

Plasmid

#101769

Purpose

Mammalian expression of TRAIP fusion protein

Depositor

Michael Huen

Article

Feng et al Cell Discov. 2016 Jun 28;2:16016. doi: 1

Insert

TRAIP / RNF206 (TRAIP Human)

Use

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-AsCas12a-2AeGFP

Plasmid

#123638

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

AsCas12a

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge