Addgene

We narrowed to 6,101 results for: donor

Showing: 1861 - 1880 of 6101 results

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pHD-mW-attPCC-FRT (pTL717)

Plasmid

#115158

Purpose

Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

mW attP-CC FRT

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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KHBD00322

Plasmid

#34447

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8591 (CTCF Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Myog'-2A-mCherryNLS-PGK-Purodtk

Plasmid

#69547

Purpose

Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Inserts

2A-mCherry-NLS
Myogenin 5' homology arm (Myog Mouse)
Myogenin 3' homology arm (Myog Mouse)
Puro-delta-tk

Use

Mouse Targeting

Tags

2x nuclear localization signal and PVT1-2A "…

Expression

Mutation

Silent V205V mutation in myogenin gene to prevent…

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5741

Plasmid

#128722

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcV-hopV1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP5910

Plasmid

#128717

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcO-hopO1-1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5058

Plasmid

#128710

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Insert

shcF-hopF2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-4xGFP KI

Plasmid

#183445

Purpose

GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and 4xGFP donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-L4-pTF-L1R

Plasmid

#45954

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

pTF promoter

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

TET optimized

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF503

Plasmid

#135735

Purpose

Donor vector for 3' FLAG tag of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 Homology arms (ZNF503 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM9121_OctaHis-GFP11

Plasmid

#153509

Purpose

OctaHis-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

OctaHis-GFP11

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF331

Plasmid

#135726

Purpose

Donor vector for 3' FLAG tag of human ZNF331

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF331 Homology arms (ZNF331 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00359

Plasmid

#34479

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7839 (CG7839 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFETCh_ZNF580

Plasmid

#86278

Purpose

Donor vector for 3' FLAG tag of human ZNF580

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF580 homology arms (ZNF580 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_SAP130

Plasmid

#86267

Purpose

Donor vector for 3' FLAG tag of human SAP130

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SAP130 homology arms (SAP130 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00140

Plasmid

#34294

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5093 (Doc3 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00175

Plasmid

#34328

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5187 (Doc2 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAWV

Plasmid

#62710

Purpose

Donor vector for amiR-eGFP knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

tdTomato/amiR-eGFP123/amiR-eGFP419/FLPe

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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KHBD00099

Plasmid

#34254

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG14711 (CG14711 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00135

Plasmid

#34289

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG30431 (CG30431 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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